ArticleNature communications2020
High-speed atomic force microscopy highlights new molecular mechanism of daptomycin action.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 60 citations in OpenAlex.
- Submolecular video-imaging of the Smc5/6 complex topologically bound to DNA.Nature communications · 2026Article
- Deep-Learning-Assisted SICM for Enhanced Real-Time Imaging of Nanoscale Biological Dynamics.Small methods · 2025Article
- A high signal-to-noise ratio and high-frequency seesaw cantilever for high-speed atomic force microscopy.Nature communications · 2025Article
- Exploring bacteria-surface interactions with a fluorescent membrane tension probe.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Nano-Scale Video Imaging of Motility Machinery by High-Speed Atomic Force Microscopy.Biomolecules · 2025Review
- Metabolic engineering of Streptomyces roseosporus for increased production of clinically important antibiotic daptomycin.Microbial biotechnology · 2024Article
- Membrane-Active Antibiotics Affect Domains in Bacterial Membranes as the First Step of Their Activity.Nano letters · 2024Article
- Article
- Hydantoin derivative dimers as broad-spectrum antimicrobial agents against ESKAPE pathogens with enhanced killing rate and stability.RSC medicinal chemistry · 2024Article
- Dissecting cell membrane tension dynamics and its effect on Piezo1-mediated cellular mechanosensitivity using force-controlled nanopipettes.Nature methods · 2024Article
- Recent Advances in Amphipathic Peptidomimetics as Antimicrobial Agents to Combat Drug Resistance.Molecules (Basel, Switzerland) · 2024Review
- Tigecycline Sensitivity Reduction inMicroorganisms · 2023Article
- Technical advances in high-speed atomic force microscopy.Biophysical reviews · 2023Review
- Visualizing the membrane disruption action of antimicrobial peptides by cryo-electron tomography.Nature communications · 2023Article
- Lateral membrane organization as target of an antimicrobial peptidomimetic compound.Nature communications · 2023Article
- Spatiotemporal resolution in high-speed atomic force microscopy for studying biological macromolecules in action.Microscopy (Oxford, England) · 2023Review
- Seeing the unseen: High-resolution AFM imaging captures antibiotic action in bacterial membranes.Nature communications · 2022Article
- Breeding of High Daptomycin-Producing Strain by Streptomycin Resistance Superposition.Polish journal of microbiology · 2022Article
- Transcriptional Regulator DasR Represses Daptomycin Production through Both Direct and Cascade Mechanisms inAntibiotics (Basel, Switzerland) · 2022Article
- A Comparison of the Adaptive Response ofFrontiers in microbiology · 2022Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The increase in speed of the high-speed atomic force microscopy (HS-AFM) compared to that of the conventional AFM made possible the first-ever visualisation at the molecular-level of the activity of an antimicrobial peptide on a membrane. We investigated the medically prescribed but poorly understood lipopeptide Daptomycin under infection-like conditions (37 °C, bacterial lipid composition and antibiotic concentrations). We confirmed so far hypothetical models: Dap oligomerization and the existence of half pores. Moreover, we detected unknown molecular mechanisms: new mechanisms to form toroidal pores or to resist Dap action, and to unprecedently quantify the energy profile of interacting oligomers. Finally, the biological and medical relevance of the findings was ensured by a multi-scale multi-nativeness-from the molecule to the cell-correlation of molecular-level information from living bacteria (Bacillus subtilis strains) to liquid-suspended vesicles and supported-membranes using electron and optical microscopies and the lipid tension probe FliptR, where we found that the cells with a healthier state of their cell wall show smaller membrane deformations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.