ArticleScience advances2020
Visualizing insulin vesicle neighborhoods in β cells by cryo-electron tomography.
Article in Science advances, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The trial behind it
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Who cites it
22 citing papers in PubMed, 39 citations in OpenAlex.
- Islet ultrastructure: past achievements and future directions.Diabetologia · 2026Review
- Classifying biophysical subpopulations of insulin secretory granules using quantitative whole-cell structure analysis.Structure (London, England : 1993) · 2026Article
- Dielectrophoresis reveals stimulus-induced remodeling of insulin granule subpopulations.Biophysical journal · 2026Article
- Mitochondria-associated programmed cell death in pancreatic β cell of T2DM.Apoptosis : an international journal on programmed cell death · 2026Review
- Integrated biophysical and spatial remodeling during insulin secretory granule maturation at the mitochondrial network.bioRxiv : the preprint server for biology · 2026Article
- Secretory stimuli distinctly regulate insulin secretory granule maturation through structural remodeling.Structure (London, England : 1993) · 2025Article
- Dielectrophoresis Reveals Stimulus-Induced Remodeling of Insulin Granule Subpopulations.bioRxiv : the preprint server for biology · 2025Article
- Classifying Biophysical Subpopulations of Insulin Secretory Granules using Quantitative Whole Cell Structure Analysis.bioRxiv : the preprint server for biology · 2025Article
- Structural glycobiology - from enzymes to organelles.Biochemical Society transactions · 2025Review
- Article
- Dysregulated inter-mitochondrial crosstalk in glioblastoma cells revealed by in situ cryo-electron tomography.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- In situ structure of actin remodeling during glucose-stimulated insulin secretion using cryo-electron tomography.Nature communications · 2024Article
- Advanced Imaging Techniques for the Characterization of Subcellular Organelle Structure in Pancreatic Islet β Cells.Comprehensive Physiology · 2023Article
- Mechanisms of gut microbiota-immune-host interaction on glucose regulation in type 2 diabetes.Frontiers in microbiology · 2023Review
- Integrative modeling of the cell.Acta biochimica et biophysica Sinica · 2022Review
- Quantitative, in situ visualization of intracellular insulin vesicles in pancreatic beta cells.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Automated vitrification of cryo-EM samples with controllable sample thickness using suction and real-time optical inspection.Nature communications · 2022Article
- Soft X-ray tomography to map and quantify organelle interactions at the mesoscale.Structure (London, England : 1993) · 2022Article
- An intensity-based post-processing tool for 3D instance segmentation of organelles in soft X-ray tomograms.PloS one · 2022Article
- Integrative structural modelling and visualisation of a cellular organelle.QRB discovery · 2022Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Subcellular neighborhoods, comprising specific ratios of organelles and proteins, serve a multitude of biological functions and are of particular importance in secretory cells. However, the role of subcellular neighborhoods in insulin vesicle maturation is poorly understood. Here, we present single-cell multiple distinct tomogram acquisitions of β cells for in situ visualization of distinct subcellular neighborhoods that are involved in the insulin vesicle secretory pathway. We propose that these neighborhoods play an essential role in the specific function of cellular material. In the regions where we observed insulin vesicles, a measurable increase in both the fraction of cellular volume occupied by vesicles and the average size (diameter) of the vesicles was apparent as sampling moved from the area near the nucleus toward the plasma membrane. These findings describe the important role of the nanometer-scale organization of subcellular neighborhoods on insulin vesicle maturation.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.