ArticleParasites & vectors2020
Wolbachia infection in wild mosquitoes (Diptera: Culicidae): implications for transmission modes and host-endosymbiont associations in Singapore.
Article in Parasites & vectors, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 26 citations in OpenAlex.
- Ecotypes,bioRxiv : the preprint server for biology · 2026Article
- Culturable microbiota of reared mosquito fauna in Osogbo, Nigeria and their potential biological roles: a molecular insight.BMC research notes · 2026Article
- Molecular detection of Wolbachia sp. and Cytoplasmic incompatibility factors (CifA/B) in wild caught mosquitoes in Côte d'Ivoire.Molecular biology reports · 2025Article
- Genetic diversity and Wolbachia infection in the Japanese encephalitis virus vector Culex tritaeniorhynchus in the Republic of Korea.Parasites & vectors · 2024Article
- Mosquito Gut Microbiota: A Review.Pathogens (Basel, Switzerland) · 2024Review
- Review
- Population genetic structure of Culex tritaeniorhynchus in different types of climatic zones in China.BMC genomics · 2024Article
- Mitochondrial and microbial diversity of the invasive mosquito vector speciesWellcome open research · 2024Article
- Aedes albopictus microbiome derives from environmental sources and partitions across distinct host tissues.MicrobiologyOpen · 2023Article
- Characterizing the Wolbachia infection in field-collected Culicidae mosquitoes from Hainan Province, China.Parasites & vectors · 2023Article
- Genome-wide detection ofFrontiers in cellular and infection microbiology · 2023Article
- SymbioticFrontiers in microbiology · 2023Review
- A tangled threesome: understanding arbovirus infection inFrontiers in microbiology · 2023Review
- Mosquito Microbiome Diversity Varies Along a Landscape-Scale Moisture Gradient.Microbial ecology · 2022Article
- Multiple long-range host shifts of major Wolbachia supergroups infecting arthropods.Scientific reports · 2022Article
- Article
- Mosquito Trilogy: Microbiota, Immunity and Pathogens, and Their Implications for the Control of Disease Transmission.Frontiers in microbiology · 2021Review
- Entomo-virological surveillance of arboviruses in the Americas: prospects for the use of paper-based nucleic acid stabilization materials.Therapeutic advances in infectious diseaseReview
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
backgroundWolbachia are intracellular bacterial endosymbionts found in most insect lineages. In mosquitoes, the influence of these endosymbionts on host reproduction and arboviral transmission has spurred numerous studies aimed at using Wolbachia infection as a vector control technique. However, there are several knowledge gaps in the literature and little is known about natural Wolbachia infection across species, their transmission modes, or associations between various Wolbachia lineages and their hosts. This study aims to address these gaps by exploring mosquito-Wolbachia associations and their evolutionary implications.
methodsWe conducted tissue-specific polymerase chain reaction screening for Wolbachia infection in the leg, gut and reproductive tissues of wild mosquitoes from Singapore using the Wolbachia surface protein gene (wsp) molecular marker. Mosquito-Wolbachia associations were explored using three methods-tanglegram, distance-based, and event-based methods-and by inferred instances of vertical transmission and host shifts.
resultsAdult mosquitoes (271 specimens) representing 14 genera and 40 species were screened for Wolbachia. Overall, 21 species (51.2%) were found positive for Wolbachia, including five in the genus Aedes and five in the genus Culex. To our knowledge, Wolbachia infections have not been previously reported in seven of these 21 species: Aedes nr. fumidus, Aedes annandalei, Uranotaenia obscura, Uranotaenia trilineata, Verrallina butleri, Verrallina sp. and Zeugnomyia gracilis. Wolbachia were predominantly detected in the reproductive tissues, which is an indication of vertical transmission. However, Wolbachia infection rates varied widely within a mosquito host species. There was no clear signal of cophylogeny between the mosquito hosts and the 12 putative Wolbachia strains observed in this study. Host shift events were also observed.
conclusionsOur results suggest that the mosquito-Wolbachia relationship is complex and that combinations of transmission modes and multiple evolutionary events likely explain the observed distribution of Wolbachia diversity across mosquito hosts. These findings have implications for a better understanding of the diversity and ecology of Wolbachia and for their utility as biocontrol agents.
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