Evidence map›Paper›PMID 33297958›Full record

ArticleBMC molecular and cell biology2020

Three functional mutation sites affect the immune response of pigs through altering the expression pattern and IgV domain of the CD4 protein.

Weiya Zhang, Juan Ni, Jie Zhang, Lu Zhang, Huanhuan Zhou, Changzhi Zhao, Mengjin Zhu, Haiyan Wang, Jianlin Han, Xinyun Li and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC molecular and cell biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact, top 84% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 2 countries.

Weiya ZhangMountainous Area Research Institute of Hebei Province, Hebei Agricultural University, No. 289 Lingyusi Street, Lianchi District, Baoding, Hebei Province, 071001, P. R. China.
Juan NiZhejiang Cancer Research Institute, Hangzhou, China.
Jie ZhangKey Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of the Ministry of Education, Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, No.1 Shizishan Street, Hongshan District, Wuhan, Hubei Province, 430070, P. R. China.
Lu ZhangKey Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of the Ministry of Education, Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, No.1 Shizishan Street, Hongshan District, Wuhan, Hubei Province, 430070, P. R. China.
Huanhuan ZhouKey Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of the Ministry of Education, Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, No.1 Shizishan Street, Hongshan District, Wuhan, Hubei Province, 430070, P. R. China.
Changzhi ZhaoKey Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of the Ministry of Education, Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, No.1 Shizishan Street, Hongshan District, Wuhan, Hubei Province, 430070, P. R. China.
Mengjin ZhuKey Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of the Ministry of Education, Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, No.1 Shizishan Street, Hongshan District, Wuhan, Hubei Province, 430070, P. R. China.
Haiyan WangKey Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of the Ministry of Education, Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, No.1 Shizishan Street, Hongshan District, Wuhan, Hubei Province, 430070, P. R. China.
Jianlin HanLivestock Genetics Program, International Livestock Research Institute (ILRI), Nairobi, Kenya.
Xinyun LiKey Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of the Ministry of Education, Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, No.1 Shizishan Street, Hongshan District, Wuhan, Hubei Province, 430070, P. R. China. xyli@mail.hzau.edu.cn.
Shuhong ZhaoKey Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of the Ministry of Education, Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, No.1 Shizishan Street, Hongshan District, Wuhan, Hubei Province, 430070, P. R. China. shzhao@mail.hzau.edu.cn.
Huazhong Agricultural University · CNMinistry of Agriculture and Rural Affairs · CNHebei Agricultural University · CNInternational Livestock Research Institute · KE

Funding

Fund of Modern Industrial Technology System of Pig CARS-35National Natural Science Foundation of China 31941008
6 · The paper itself

Abstract

backgroundThe CD4 protein is an important surface marker of T lymphocytes, which can mediate the antigen presentation process by interacting with MHC II and TCR molecules in human and mouse.

resultsIn this study, two haplotypes (A and B) of the CD4 gene were found within Chinese indigenous and Western commercial pig breeds. These two haplotypes were defined by 22 fully linked SNPs in the CDS region of the CD4 gene. The expression level and localization of the CD4 protein were significantly different between haplotypes A and B. Transcriptome analysis revealed that the immune response-related genes and signaling pathways were down-regulated in genotype AA. Finally, three linked functional SNPs were identified, which affected the expression level and membrane localization of the CD4 protein in pigs. These three SNPs led to the replacements of two amino acids in the IgV1 domain of the CD4 protein, and related to the function of the CD4 protein in the immune response.

conclusionThese three linked SNPs were the key functional mutation sites in the CD4 gene, which played important roles in the immune response, and could be utilized as new molecular markers in breeding for disease resistance in pigs.

Indexed as

AllelesAmino Acid SequenceAnimalsBase SequenceBreedingCD4 AntigensCell MembraneHaplotypesImmunityMutationPolymorphism, Single NucleotideProtein DomainsSwineCD4 AntigensCD4 geneFunctional mutationsImmune responseMembrane localizationPigTranslation

Identifiers

PMID33297958
PMCPMC7724863
OpenAlexW3113268142

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.