ArticleJournal of microscopy2022
Sensitivity of remote focusing microscopes to magnification mismatch.
Article in Journal of microscopy, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Rapid and gentle volumetric imaging of host-pathogen interactions in salmon skin cells using projective oblique plane microscopy.Scientific reports · 2025Article
- Cleared tissue dual-view oblique plane microscopy.Biomedical optics express · 2025Article
- Remote refocusing for multi-scale imaging.Journal of biomedical optics · 2024Article
- Axial de-scanning using remote focusing in the detection arm of light-sheet microscopy.Nature communications · 2024Article
- User-friendly oblique plane microscopy on a fully functional commercially available microscope base.Biomedical optics express · 2024Article
- S-polarized light-sheets improve resolution and light-efficiency in oblique plane microscopy.Scientific reports · 2024Article
- Axial de-scanning using remote focusing in the detection arm of light-sheet microscopy.Research square · 2023Article
- Alignment and characterization of remote-refocusing systems.Applied optics · 2023Article
- Sensitivity of remote focusing microscopes to magnification mismatch.Journal of microscopy · 2022Article
- A fully water coupled oblique light-sheet microscope.Scientific reports · 2022Article
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Remote focusing (RF) is a technique that greatly extends the aberration-free axial scan range of an optical microscope. To maximise the diffraction limited depth range in an RF system, the magnification of the relay lenses should be such that the pupil planes of the objectives are accurately mapped on to each other. In this paper we study the tolerance of the RF system to magnification mismatch and quantify the amount of residual spherical aberration present at different focusing depths. We observe that small deviations from ideal magnification results in increased amounts of residual spherical aberration terms leading to a reduction in the diffracted limited range. For high-numerical aperture objectives, the simulation predicts a 50% decrease in the diffracted limited range for 1% magnification mismatch. The simulation has been verified against an experimental RF system with ideal and nonideal magnifications. Experimentally confirmed predictions also provide a valuable empirical method of determining when a system is close to the ideal phase matching condition, based on the sign of the spherical aberration on either side of focus.
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