Evidence map›Paper›PMID 33295147›Full record

ArticleChemMedChem2021

Dual-Mechanism Quenched Fluorogenic Probe Provides Selective and Rapid Detection of Cathepsin L Activity*.

Kelton A Schleyer, Ben Fetrow, Peter Zannes Fatland, Jun Liu, Maya Chaaban, Biwu Ma, Lina Cui

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ChemMedChem, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Kelton A SchleyerDepartment of Medicinal Chemistry, UF Health Science Center, UF Health Cancer Center, University of Florida, 1345 Center Dr., Gainesville, FL 32610, USA.
Ben FetrowDepartment of Chemistry and Chemical Biology, UNM Comprehensive Cancer Center, University of New Mexico, 300 Terrace St. NE, Albuquerque, NM 87131, USA.
Peter Zannes FatlandDepartment of Chemistry and Chemical Biology, UNM Comprehensive Cancer Center, University of New Mexico, 300 Terrace St. NE, Albuquerque, NM 87131, USA.
Jun LiuDepartment of Medicinal Chemistry, UF Health Science Center, UF Health Cancer Center, University of Florida, 1345 Center Dr., Gainesville, FL 32610, USA.
Maya ChaabanDepartment of Chemistry and Biochemistry, Florida State University, 95 Chieftan Way 118 DLC, Tallahassee, FL 32306, USA.
Biwu MaDepartment of Chemistry and Biochemistry, Florida State University, 95 Chieftan Way 118 DLC, Tallahassee, FL 32306, USA.
Lina CuiDepartment of Medicinal Chemistry, UF Health Science Center, UF Health Cancer Center, University of Florida, 1345 Center Dr., Gainesville, FL 32610, USA.ORCID 0000-0002-8149-5206

Funding

WOMEN'S CANCERS RESEARCH PROGRAMP30CA118100 · NCI · UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR · PI Yolanda Sanchez · 2005 to 2026
$57.1M
Probing the role of heparanase via in situ labelingR35GM124963 · NIGMS · UNIVERSITY OF NEW MEXICO · PI CUI, LINA · 2017 to 2021
$1.9M
Acquisition of a Q Exactive HF Hybrid Quadrupole-OrbitrapS10OD021758 · OD · UNIVERSITY OF FLORIDA · PI BASSO, KARI B · 2017 to 2017
$600k
Department of Defense Career Development Award W81XWH-17-1-0529Department of Medicinal Chemistry, College of Pharmacy, University of Florida (UF)Mass Spectrometry Facility, Department of Chemistry and Chemical Biology, UNMNational Cancer Institute of the United States P30CA118100NCI NIH HHS P30 CA118100NIGMS NIH HHS R35 GM124963NIH HHS S10 OD021758NMR Facility, Department of Chemistry and Chemical Biology, UNMUniversity of New Mexico (UNM)UNM Comprehensive Cancer Center
6 · The paper itself

Abstract

Cathepsin L (CTL) is a cysteine protease demonstrating upregulated activity in many disease states. Overlapping substrate specificity makes selective detection of CTL activity difficult to parse from that of its close homologue CTV and the ubiquitous CTB. Current probes of CTL activity have limited applications due to either poor contrast or extra assay steps required to achieve selectivity. We have developed a fluorogenic probe, CTLAP, that displays good selectivity for CTL over CTB and CTV while exhibiting low background fluorescence attributed to dual quenching mechanisms. CTLAP achieves optimum CTL selectivity in the first 10 min of incubation, thus suggesting that it is amenable for rapid detection of CTL, even in the presence of competing cathepsins.

Indexed as

Cathepsin LDensity Functional TheoryFluorescent DyesHumansMolecular StructureCathepsin LCTSL protein, humanFluorescent DyesCathepsinsenzymesfluorescent probesmedicinal chemistryquenching

Identifiers

PMID33295147
PMCPMC8202353

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.