ArticleScientific reports2020
GRAS-Di system facilitates high-density genetic map construction and QTL identification in recombinant inbred lines of the wheat progenitor Aegilops tauschii.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed.
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- Establishment of a Non-transgenic Iron-Biofortified Rice Line Using a Novel HRZ1 Mutation.Rice (New York, N.Y.) · 2026Article
- Extensive phenotyping and genome-wide analysis of grain number-related traits in a diverse wheat core collection.The plant genome · 2026Article
- Evolution and functioning of an X-A balance sex-determining system in hops.Nature plants · 2025Article
- Machine learning for genomic and pedigree prediction in sugarcane.The plant genome · 2024Article
- Discrepancy of flowering time between genetically close sublineages of Aegilops umbellulata Zhuk.Scientific reports · 2024Article
- Heat Stress-Tolerant Quantitative Trait Loci Identified Using Backcrossed Recombinant Inbred Lines Derived from Intra-Specifically DiversePlants (Basel, Switzerland) · 2024Article
- Quantitative trait locus (QTL) analysis and fine-mapping forBreeding science · 2023Article
- Chromosome-scale genome assembly of Eustoma grandiflorum, the first complete genome sequence in the genus Eustoma.G3 (Bethesda, Md.) · 2023Article
- Utilization of Genotyping-by-Sequencing (GBS) for Rice Pre-Breeding and Improvement: A Review.Life (Basel, Switzerland) · 2022Review
- A Survey of Genome-Wide Genetic Characterizations of Crossbred Dairy Cattle in Local Farms in Cambodia.Animals : an open access journal from MDPI · 2022Article
- Genetic basis of maturity time is independent from that of flowering time and contributes to ecotype differentiation in common buckwheat (Fagopyrum esculentum Moench).BMC plant biology · 2022Article
- Developing core marker sets for effective genomic-assisted selection in wheat and barley breeding programs.Breeding science · 2022Article
- MIG-seq is an effective method for high-throughput genotyping in wheat (Triticum spp.).DNA research : an international journal for rapid publication of reports on genes and genomes · 2022Article
- Recombinant inbred lines and next-generation sequencing enable rapid identification of candidate genes involved in morphological and agronomic traits in foxtail millet.Scientific reports · 2022Article
- A Major and Stable Quantitative Trait LocusFrontiers in genetics · 2021Article
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8 authors.
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Abstract
Due to large and complex genomes of Triticeae species, skim sequencing approaches have cost and analytical advantages for detecting genetic markers and building linkage maps. Here, we develop a high-density linkage map and identify quantitative trait loci (QTLs) for recombinant inbred lines of Aegilops tauschii, a D-genome donor of bread wheat, using the recently developed genotyping by Random Amplicon Sequencing-Direct (GRAS-Di) system, which facilitates skimming of the large and complicated genome and generates a large number of genetic markers. The deduced linkage groups based on the GRAS-Di genetic markers corresponded to the chromosome number of Ae. tauschii. We successfully identified stable QTLs for flowering time and spikelet shape-related traits. Genotype differences of RILs at the QTL-linked markers were significantly associated with the trait variations. In particular, one of the QTL-linked markers for flowering time was mapped close to VRN3 (also known as FLOWERING LOCUS T), which controls flowering. The GRAS-Di system is, therefore, an efficient and useful application for genotyping and linkage mapping in species with large and complex genomes, such as Triticeae species.
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