Evidence map›Paper›PMID 33293367›Full record

ArticleJournal of clinical microbiology2021

A Fast and Accessible Method for the Isolation of RNA, DNA, and Protein To Facilitate the Detection of SARS-CoV-2.

Jose Carlos Ponce-Rojas, Michael S Costello, Duncan A Proctor, Kenneth S Kosik, Maxwell Z Wilson, Carolina Arias, Diego Acosta-Alvear

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of clinical microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
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  3. Review
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  8. Article
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  10. Article
  11. Frugal Science Powered by Curiosity.Industrial & engineering chemistry research · 2021
    Article
  12. Review
  13. Nucleic Acid Testing of SARS-CoV-2.International journal of molecular sciences · 2021
    Review
  14. Article
  15. Article
  16. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Jose Carlos Ponce-Rojas *Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, Santa Barbara, California, USA.ORCID 0000-0002-0925-3990
Michael S Costello *Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, Santa Barbara, California, USA.ORCID 0000-0003-4132-6966
Duncan A ProctorDepartment of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, Santa Barbara, California, USA.
Kenneth S KosikDepartment of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, Santa Barbara, California, USA.ORCID 0000-0003-3224-5179
Maxwell Z WilsonDepartment of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, Santa Barbara, California, USA.ORCID 0000-0003-0768-7004
Carolina Arias *Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, Santa Barbara, California, USA carolinaarias@ucsb.edu daa@lifesci.ucsb.edu.ORCID 0000-0002-4445-0826
Diego Acosta-Alvear *Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, Santa Barbara, California, USA carolinaarias@ucsb.edu daa@lifesci.ucsb.edu.ORCID 0000-0002-1139-8486

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Management of the coronavirus disease 2019 (COVID-19) pandemic requires widespread testing for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). A main limitation for widespread SARS-CoV-2 testing is the global shortage of essential supplies, among them RNA extraction kits. The need for commercial RNA extraction kits places a bottleneck on tests that detect SARS-CoV-2 genetic material, including PCR-based reference tests. Here, we propose an alternative method we call PEARL (

Indexed as

COVID-19SARS-CoV-2Clinical Laboratory TechniquesCOVID-19 TestingDNAHumansRNA, ViralDNARNA, ViralDNA and proteinfield deployablerapid isolation of RNASARS-CoV-2virus detection

Identifiers

PMID33293367
PMCPMC8092744

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.