ArticleBiotechnology for biofuels2020
LACCASE14 is required for the deposition of guaiacyl lignin and affects cell wall digestibility in poplar.
Article in Biotechnology for biofuels, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 62 citations in OpenAlex.
- Heterologous expression of Pinus massoniana VNS2 enhances secondary cell wall deposition in poplar.Plant cell reports · 2026Article
- The laccase geneFrontiers in plant science · 2026Article
- Deciphering the underlying genetics of galling resistance to the blueberry stem gall wasp in northern highbush blueberry.Horticulture research · 2025Article
- Factors underlying a latitudinal gradient in the S/G lignin monomer ratio in natural poplar variants.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Telomere-to-telomere, gap-free genome of mung bean (Horticulture research · 2025Article
- The ASYMMETRIC LEAVES1 ortholog PagAS1a promotes xylem development and plant growth inForestry research · 2025Article
- Genome-Wide Identification and Characterization of the Laccase Gene Family inPlants (Basel, Switzerland) · 2024Article
- Applications of CRISPR Technologies in Forestry and Molecular Wood Biotechnology.International journal of molecular sciences · 2024Review
- Article
- Transcriptome and miRNAs Profiles Reveal Regulatory Network and Key Regulators of Secondary Xylem Formation in "84K" Poplar.International journal of molecular sciences · 2023Article
- Transcriptome and Low-Affinity Sodium Transport Analysis Reveals Salt Tolerance Variations between Two Poplar Trees.International journal of molecular sciences · 2023Article
- Lignin engineering in forest trees: From gene discovery to field trials.Plant communications · 2022Review
- Overexpression of PtoMYB115 improves lignocellulose recalcitrance to enhance biomass digestibility and bioethanol yield by specifically regulating lignin biosynthesis in transgenic poplar.Biotechnology for biofuels and bioproducts · 2022Article
- Genome-Wide Identification of Switchgrass Laccases Involved in Lignin Biosynthesis and Heavy-Metal Responses.International journal of molecular sciences · 2022Article
- Recent advances in metabolic engineering of microorganisms for advancing lignocellulose-derived biofuels.Bioengineered · 2022Review
- Regulation of PaRBOH1-mediated ROS production in Norway spruce by CaFrontiers in plant science · 2022Article
- MdMYB52 regulates lignin biosynthesis upon the suberization process in apple.Frontiers in plant science · 2022Article
- LigninFrontiers in plant science · 2022Article
- Spatio-Temporal Modification of Lignin Biosynthesis in Plants: A Promising Strategy for Lignocellulose Improvement and Lignin Valorization.Frontiers in bioengineering and biotechnology · 2022Review
- The role of JrLACs in the lignification of walnut endocarp.BMC plant biology · 2021Article
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7 authors at 1 institution in 1 country.
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Abstract
backgroundThe recalcitrance of lignocellulosic biomass provided technical and economic challenges in the current biomass conversion processes. Lignin is considered as a crucial recalcitrance component in biomass utilization. An in-depth understanding of lignin biosynthesis can provide clues to overcoming the recalcitrance. Laccases are believed to play a role in the oxidation of lignin monomers, leading to the formation of higher-order lignin. In plants, functions of only a few laccases have been evaluated, so little is known about the effect of laccases on cell wall structure and biomass saccharification.
resultsIn this study, we screened a gain-of-function mutant with a significant increase in lignin content from Arabidopsis mutant lines overexpressing a full-length poplar cDNA library. Further analysis confirmed that a Chinese white poplar (Populus tomentosa) laccase gene PtoLAC14 was inserted into the mutant, and PtoLAC14 could functionally complement the Arabidopsis lac4 mutant. Overexpression of PtoLAC14 promoted the lignification of poplar and reduced the proportion of syringyl/guaiacyl. In contrast, the CRISPR/Cas9-generated mutation of PtLAC14 results in increased the syringyl/guaiacyl ratios, which led to integrated enhancement on biomass enzymatic saccharification. Notably, the recombinant PtoLAC14 protein showed higher oxidized efficiency to coniferyl alcohol (precursor of guaiacyl unit) in vitro.
conclusionsThis study shows that PtoLAC14 plays an important role in the oxidation of guaiacyl deposition on cell wall. The reduced recalcitrance of the PtoLAC14-KO lines suggests that PtoLAC14 is an elite target for cell wall engineering, and genetic manipulation of this gene will facilitate the utilization of lignocellulose.
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