ArticleBiotechnology for biofuels2020
Water-soluble chlorophyll-binding proteins from Brassica oleracea allow for stable photobiocatalytic oxidation of cellulose by a lytic polysaccharide monooxygenase.
Article in Biotechnology for biofuels, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 14 citations in OpenAlex.
- Effect of the nature of the photonic energy on the photochemical and physiochemical response of Nostoc commune under Cr (VI) toxicity.International microbiology : the official journal of the Spanish Society for Microbiology · 2025Article
- Cancer Photodynamic Therapy Enabled by Water-Soluble Chlorophyll Protein.ACS applied materials & interfaces · 2025Article
- Testing eukaryotic routes for heterologous production of ketocarotenoids in cyanobacteria.Sustainable microbiology · 2025Article
- Visible light-exposed lignin facilitates cellulose solubilization by lytic polysaccharide monooxygenases.Nature communications · 2023Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
Abstract
backgroundLytic polysaccharide monooxygenases (LPMOs) are indispensable redox enzymes used in industry for the saccharification of plant biomass. LPMO-driven cellulose oxidation can be enhanced considerably through photobiocatalysis using chlorophyll derivatives and light. Water soluble chlorophyll binding proteins (WSCPs) make it is possible to stabilize and solubilize chlorophyll in aqueous solution, allowing for in vitro studies on photostability and ROS production. Here we aim to apply WSCP-Chl a as a photosensitizing complex for photobiocatalysis with the LPMO, TtAA9.
resultsWe have in this study demonstrated how WSCP reconstituted with chlorophyll a (WSCP-Chl a) can create a stable photosensitizing complex which produces controlled amounts of H
conclusionWith WSCP-Chl a as the photosensitizer, the need to replenish Chl is greatly reduced, enhancing the catalytic lifetime of light-driven LPMOs and increasing the efficiency of cellulose depolymerization. WSCP-Chl a allows for stable photobiocatalysis providing a sustainable solution for biomass processing.
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