Evidence map›Paper›PMID 33292428›Full record

ArticleBiotechnology for biofuels2020

Water-soluble chlorophyll-binding proteins from Brassica oleracea allow for stable photobiocatalytic oxidation of cellulose by a lytic polysaccharide monooxygenase.

N Dodge, D A Russo, B M Blossom, R K Singh, B van Oort, R Croce, M J Bjerrum, P E Jensen

Open access · goldAbstract read
In one paragraph

Article in Biotechnology for biofuels, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.6field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 14 citations in OpenAlex.

  1. Effect of the nature of the photonic energy on the photochemical and physiochemical response of Nostoc commune under Cr (VI) toxicity.International microbiology : the official journal of the Spanish Society for Microbiology · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 3 countries.

N DodgeDepartment of Food Science, University of Copenhagen, Rolighedsvej 26, 1958, Frederiksberg, Denmark.
D A RussoDepartment of Bioorganic Analytics, Institute for Inorganic and Analytical Chemistry, Friedrich Schiller University Jena, Jena, Germany.
B M BlossomDepartment of Geosciences and Natural Resource Management, University of Copenhagen, Frederiksberg C, Denmark.
R K SinghDepartment of Chemistry, University of Copenhagen, Copenhagen, Denmark.
B van OortBiophysics of Photosynthesis, Department of Physics and Astronomy, Faculty of Sciences, and LaserLaB Amsterdam, Vrije Universiteit Amsterdam, Amsterdam, The Netherlands.
R CroceBiophysics of Photosynthesis, Department of Physics and Astronomy, Faculty of Sciences, and LaserLaB Amsterdam, Vrije Universiteit Amsterdam, Amsterdam, The Netherlands.
M J BjerrumDepartment of Chemistry, University of Copenhagen, Copenhagen, Denmark.
P E JensenDepartment of Food Science, University of Copenhagen, Rolighedsvej 26, 1958, Frederiksberg, Denmark. peje@food.ku.dk.ORCID http://orcid.org/0000-0001-6524-7723
University of Copenhagen · DKVrije Universiteit Amsterdam · NLFriedrich Schiller University Jena · DE

Funding

Novo Nordisk Fonden NNF16OC0021832Villum Fonden No. 13363
6 · The paper itself

Abstract

backgroundLytic polysaccharide monooxygenases (LPMOs) are indispensable redox enzymes used in industry for the saccharification of plant biomass. LPMO-driven cellulose oxidation can be enhanced considerably through photobiocatalysis using chlorophyll derivatives and light. Water soluble chlorophyll binding proteins (WSCPs) make it is possible to stabilize and solubilize chlorophyll in aqueous solution, allowing for in vitro studies on photostability and ROS production. Here we aim to apply WSCP-Chl a as a photosensitizing complex for photobiocatalysis with the LPMO, TtAA9.

resultsWe have in this study demonstrated how WSCP reconstituted with chlorophyll a (WSCP-Chl a) can create a stable photosensitizing complex which produces controlled amounts of H

conclusionWith WSCP-Chl a as the photosensitizer, the need to replenish Chl is greatly reduced, enhancing the catalytic lifetime of light-driven LPMOs and increasing the efficiency of cellulose depolymerization. WSCP-Chl a allows for stable photobiocatalysis providing a sustainable solution for biomass processing.

Indexed as

CelluloseChlorophyll-binding proteinLight-drivenMonooxygenasesPhotobiocatalysis

Identifiers

PMID33292428
PMCPMC7708235
OpenAlexW3108382651

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.