ArticlePlant methods2020
A comprehensive comparison of four methods for extracting lipids from Arabidopsis tissues.
Article in Plant methods, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 42 citations in OpenAlex.
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- Analytical challenges and opportunities in the extraction, separation, and identification of food phospholipids.Frontiers in nutrition · 2026Review
- Lipidomic profiling of Arabidopsis chloroplast protein phosphatase SLP1 mutants reveals altered diurnal lipid remodeling.BBA advances · 2026Article
- Biosynthesis of silver nanoparticles from macroalgae Hormophysa triquetra and investigation of its antibacterial activity and mechanism against pathogenic bacteria.Scientific reports · 2025Article
- Chia (Salvia hispanica L.), a functional 'superfood': new insights into its botanical, genetic and nutraceutical characteristics.Annals of botany · 2024Review
- Recent Analytical Methodologies in Lipid Analysis.International journal of molecular sciences · 2024Review
- De novo cholesterol biosynthesis in bacteria.Nature communications · 2023Article
- Lipidomic and transcriptomic profiles of glycerophospholipid metabolism during Hemerocallis citrina Baroni flowering.BMC plant biology · 2023Article
- Lipid remodeling of contrasting maize (Frontiers in plant science · 2023Article
- Biochemical Changes in Two Barley Genotypes Inoculated With a Beneficial FungusFrontiers in plant science · 2022Article
- An Arabidopsis lipid map reveals differences between tissues and dynamic changes throughout development.The Plant journal : for cell and molecular biology · 2021Article
- The Metabolic Response of Brachypodium Roots to the Interaction with Beneficial Bacteria Is Affected by the Plant Nutritional Status.Metabolites · 2021Article
- Three-in-One Simultaneous Extraction of Proteins, Metabolites and Lipids for Multi-Omics.Frontiers in genetics · 2021Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundThe plant lipidome is highly complex, and the composition of lipids in different tissues as well as their specific functions in plant development, growth and stress responses have yet to be fully elucidated. To do this, efficient lipid extraction protocols which deliver target compounds in solution at concentrations adequate for subsequent detection, quantitation and analysis through spectroscopic methods are required. To date, numerous methods are used to extract lipids from plant tissues. However, a comprehensive analysis of the efficiency and reproducibility of these methods to extract multiple lipid classes from diverse tissues of a plant has not been undertaken.
resultsIn this study, we report the comparison of four different lipid extraction procedures in order to determine the most effective lipid extraction protocol to extract lipids from different tissues of the model plant Arabidopsis thaliana.
conclusionWhile particular methods were best suited to extract different lipid classes from diverse Arabidopsis tissues, overall a single-step extraction method with a 24 h extraction period, which uses a mixture of chloroform, isopropanol, methanol and water, was the most efficient, reproducible and the least labor-intensive to extract a broad range of lipids for untargeted lipidomic analysis of Arabidopsis tissues. This method extracted a broad range of lipids from leaves, stems, siliques, roots, seeds, seedlings and flowers of Arabidopsis. In addition, appropriate methods for targeted lipid analysis of specific lipids from particular Arabidopsis tissues were also identified.
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