ArticleProceedings of the National Academy of Sciences of the United States of America2020
Cortical tension overrides geometrical cues to orient microtubules in confined protoplasts.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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35 citing papers in PubMed, 78 citations in OpenAlex.
- Engineering Biomimetic Sporangia in Land Plants.Bioengineering (Basel, Switzerland) · 2026Article
- The role of geometry in morphogenesis.Development (Cambridge, England) · 2026Review
- Temporal changes in surface tension guide microtubule organization and accurate asymmetric division of Arabidopsis zygotes.Nature communications · 2026Article
- 3D geometry and mechanics of a single apical stem cell ensure helically symmetric plant body in multicellular models.Frontiers in plant science · 2026Article
- Transverse cortical microtubule arrays form persistent unipolar domains in hypocotyl cells ofMolecular biology of the cell · 2025Article
- Mechanical contribution of germ cell specification inScience advances · 2025Article
- Conflicting roles of cell geometry, microtubule deflection, and orientation-dependent dynamic instability in cortical array organization.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- The Extremely Low Mechanical Force Generated by Nano-Pulling Induces Global Changes in the Microtubule Network, Nuclear Morphology, and Chromatin Transcription in Neurons.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Microtubule-based nucleation results in a large sensitivity to cell geometry of the plant cortical array.PLoS computational biology · 2025Article
- Agent-based simulation of cortical microtubule band movement in arabidopsis zygotes.Scientific reports · 2025Article
- Microtubule flexibility, microtubule-based nucleation and ROP pattern co-alignment enhance protoxylem microtubule patterning.Quantitative plant biology · 2025Article
- Open quantum systems theory of ultraweak ultraviolet photon emissions: Revisiting Gurwitsch's onion experiment as a prototype for quantum biology.Computational and structural biotechnology journal · 2024Review
- Robust organ size in Arabidopsis is primarily governed by cell growth rather than cell division patterns.Development (Cambridge, England) · 2024Article
- Mechanical forces exerted on floral primordia with a novel experimental system modify floral development in Arabidopsis thaliana.Journal of plant research · 2024Article
- Mechanical state transitions in the regulation of tissue form and function.Nature reviews. Molecular cell biology · 2024Review
- A transient radial cortical microtubule array primes cell division inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Spatiotemporally distinct responses to mechanical forces shape the developing seed of Arabidopsis.The EMBO journal · 2024Article
- The dynamics and biophysics of shape formation: Common themes in plant and animal morphogenesis.Developmental cell · 2023Review
- Microtubule-associated protein SlMAP70 interacts with IQ67-domain protein SlIQD21a to regulate fruit shape in tomato.The Plant cell · 2023Article
- Computer models of cell polarity establishment in plants.Plant physiology · 2023Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In plant cells, cortical microtubules (CMTs) generally control morphogenesis by guiding cellulose synthesis. CMT alignment has been proposed to depend on geometrical cues, with microtubules aligning with the cell long axis in silico and in vitro. Yet, CMTs are usually transverse in vivo, i.e., along predicted maximal tension, which is transverse for cylindrical pressurized vessels. Here, we adapted a microwell setup to test these predictions in a single-cell system. We confined protoplasts laterally to impose a curvature ratio and modulated pressurization through osmotic changes. We find that CMTs can be longitudinal or transverse in wallless protoplasts and that the switch in CMT orientation depends on pressurization. In particular, longitudinal CMTs become transverse when cortical tension increases. This explains the dual behavior of CMTs in planta: CMTs become longitudinal when stress levels become low, while stable transverse CMT alignments in tissues result from their autonomous response to tensile stress fluctuations.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.