Evidence map›Paper›PMID 33287848›Full record

ArticleJournal of nanobiotechnology2020

Improvement of stem cell-derived exosome release efficiency by surface-modified nanoparticles.

Dong Jun Park, Wan Su Yun, Woo Cheol Kim, Jeong-Eun Park, Su Hoon Lee, Sunmok Ha, Jin Sil Choi, Jaehong Key, Young Joon Seo

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of nanobiotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.

0numbers the graph read from it
0cells of the map it votes in
49citing papers in PubMed
4.0field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

49 citing papers in PubMed, 74 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Dong Jun ParkDepartment of Otorhinolaryngology, Yonsei University Wonju College of Medicine, 20 Ilsan-ro, Wonju, Gangwon-do, 26426, South Korea.
Wan Su YunDepartment of Biomedical Engineering, Yonsei University, Wonju, South Korea.
Woo Cheol KimDepartment of Biomedical Engineering, Yonsei University, Wonju, South Korea.
Jeong-Eun ParkDepartment of Otorhinolaryngology, Yonsei University Wonju College of Medicine, 20 Ilsan-ro, Wonju, Gangwon-do, 26426, South Korea.
Su Hoon LeeDepartment of Otorhinolaryngology, Yonsei University Wonju College of Medicine, 20 Ilsan-ro, Wonju, Gangwon-do, 26426, South Korea.
Sunmok HaDepartment of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, Republic of Korea.
Jin Sil ChoiDepartment of Otorhinolaryngology, Yonsei University Wonju College of Medicine, 20 Ilsan-ro, Wonju, Gangwon-do, 26426, South Korea.
Jaehong KeyDepartment of Biomedical Engineering, Yonsei University, Wonju, South Korea.
Young Joon SeoDepartment of Otorhinolaryngology, Yonsei University Wonju College of Medicine, 20 Ilsan-ro, Wonju, Gangwon-do, 26426, South Korea. okas2000@hanmail.net.ORCID http://orcid.org/0000-0002-2839-4676
Yonsei University · KR

Funding

Korea Health Industry Development Institute (KR) HI19C1334Ministry of SMEs and Startups Project No. S2921850
6 · The paper itself

Abstract

backgroundMesenchymal stem cells (MSCs) are pluripotent stromal cells that release extracellular vesicles (EVs). EVs contain various growth factors and antioxidants that can positively affect the surrounding cells. Nanoscale MSC-derived EVs, such as exosomes, have been developed as bio-stable nano-type materials. However, some issues, such as low yield and difficulty in quantification, limit their use. We hypothesized that enhancing exosome production using nanoparticles would stimulate the release of intracellular molecules.

resultsThe aim of this study was to elucidate the molecular mechanisms of exosome generation by comparing the internalization of surface-modified, positively charged nanoparticles and exosome generation from MSCs. We determined that Rab7, a late endosome and auto-phagosome marker, was increased upon exosome expression and was associated with autophagosome formation.

conclusionsIt was concluded that the nanoparticles we developed were transported to the lysosome by clathrin-mediated endocytosis. additionally, entered nanoparticles stimulated that autophagy related factors to release exosome from the MSC. MSC-derived exosomes using nanoparticles may increase exosome yield and enable the discovery of nanoparticle-induced genetic factors.

Indexed as

ExosomesMesenchymal Stem CellsAnimalsAutophagyCells, CulturedMagnetite NanoparticlesMiceMicroRNAsPolyethyleneiminePolylactic Acid-Polyglycolic Acid Copolymerrab7 GTP-Binding Proteinsrab GTP-Binding ProteinsSurface PropertiesMagnetite NanoparticlesMicroRNAsPolyethyleneiminePolylactic Acid-Polyglycolic Acid Copolymerrab7 GTP-Binding Proteinsrab7 GTP-binding proteins, mouserab GTP-Binding ProteinsAutophagyMesenchymal stem cell-derived exosomePLGA-PEI NPsPositively charged nanoparticleRab7

Identifiers

PMID33287848
PMCPMC7720507
OpenAlexW3082499954

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.