Evidence map›Paper›PMID 33287721›Full record

ArticleBMC plant biology2020

Linkage mapping and QTL analysis of flowering time using ddRAD sequencing with genotype error correction in Brassica napus.

Armin Scheben, Anita A Severn-Ellis, Dhwani Patel, Aneeta Pradhan, Stephen J Rae, Jacqueline Batley, David Edwards

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC plant biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
0.7field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 17 citations in OpenAlex.

  1. Candidate genes for stem rust resistance in Italian ryegrass revealed by nested association mapping.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026
    Article
  2. A key gene for vernalization-independent bolting under extra-long days in biennial sugar beet.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026
    Article
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  4. Review
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  7. Structural variations and environmental specificities of flowering time-related genes in Brassica napus.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 3 countries.

Armin SchebenSchool of Biological Sciences and Institute of Agriculture, The University of Western Australia, Perth, WA, Australia.
Anita A Severn-EllisSchool of Biological Sciences and Institute of Agriculture, The University of Western Australia, Perth, WA, Australia.
Dhwani PatelSchool of Biological Sciences and Institute of Agriculture, The University of Western Australia, Perth, WA, Australia.
Aneeta PradhanSchool of Biological Sciences and Institute of Agriculture, The University of Western Australia, Perth, WA, Australia.
Stephen J RaeBASF Agricultural Solutions Belgium NV, BASF Innovation Center Gent, Technologiepark-Zwijnaarde 101, 9052, Ghent, Belgium.
Jacqueline BatleySchool of Biological Sciences and Institute of Agriculture, The University of Western Australia, Perth, WA, Australia.
David EdwardsSchool of Biological Sciences and Institute of Agriculture, The University of Western Australia, Perth, WA, Australia. dave.edwards@uwa.edu.au.ORCID http://orcid.org/0000-0001-7599-6760
The University of Western Australia · AUBio Base Europe · BE

Funding

Australian Research Council LP130100925Australian Research Council LP140100537Australian Research Council LP160100030
6 · The paper itself

Abstract

backgroundBrassica napus is an important oilseed crop cultivated worldwide. During domestication and breeding of B. napus, flowering time has been a target of selection because of its substantial impact on yield. Here we use double digest restriction-site associated DNA sequencing (ddRAD) to investigate the genetic basis of flowering in B. napus. An F

resultsFlowering time in the mapping population differed by up to 25 days between individuals. High genotype error rates persisted after initial quality controls, as suggested by a genotype discordance of ~ 12% between biological sequencing replicates. After genotype error correction, a linkage map spanning 3981.31 cM and compromising 14,630 single nucleotide polymorphisms (SNPs) was constructed. A quantitative trait locus (QTL) on chromosome C2 was detected, covering eight flowering time genes including FLC.

conclusionsThese findings demonstrate the effectiveness of the ddRAD approach to sample the B. napus genome. Our results also suggest that ddRAD genotype error rates can be higher than expected in F

Indexed as

AllelesBinding SitesBrassica napusChromosome MappingChromosomes, PlantDNA Restriction EnzymesFlowersGenes, PlantGenome, PlantGenotypePhenotypePolymorphism, Single NucleotideQuantitative Trait LociSequence Analysis, DNATime FactorsDNA Restriction EnzymesBrassica napusDouble digest restriction-site associated DNA sequencingFloweringQTLSNP

Identifiers

PMID33287721
PMCPMC7720618
OpenAlexW3110783737

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.