ArticleBMC genomics2020
Genome-wide characterization, expression analyses, and functional prediction of the NPF family in Brassica napus.
Article in BMC genomics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Genome-wide analyses of NPF and NRT2 reveal a potential role for a key gene, CqNRT2.2, in the quinoa response to nitrate.BMC plant biology · 2026Article
- Comprehensive genome-wide identification of the NPF gene family and functional characterization of GmNPF6.8 regulating root development in soybean.BMC plant biology · 2026Article
- Characterization ofFrontiers in plant science · 2026Article
- Insights into the ameliorative effect of ZnONPs on arsenic toxicity in soybean mediated by hormonal regulation, transporter modulation, and stress responsive genes.Frontiers in plant science · 2024Article
- Genome-wide identification and expression analysis of the NRT genes in Ginkgo biloba under nitrate treatment reveal the potential roles during calluses browning.BMC genomics · 2023Article
- Genome-Wide Identification and Expression Analysis ofPlants (Basel, Switzerland) · 2023Article
- Exploring the genetic landscape of nitrogen uptake in durum wheat: genome-wide characterization and expression profiling of NPF and NRT2 gene families.Frontiers in plant science · 2023Article
- Identification ofInternational journal of molecular sciences · 2023Article
- Transcriptome and metabolome profiling of interspecific CSSLs reveals general and specific mechanisms of drought resistance in cotton.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Systematic Investigation and Expression Profiles of the Nitrate Transporter 1/Peptide Transporter Family (NPF) in Tea Plant (International journal of molecular sciences · 2022Article
- Phylogeny and Expression Atlas of thePlants (Basel, Switzerland) · 2022Article
- De novo transcriptome assembly and analysis of gene expression in different tissues of moth bean (Vigna aconitifolia) (Jacq.) Marechal.BMC plant biology · 2022Article
- The impacts of allopolyploidization on Methyl-CpG-Binding Domain (MBD) gene family in Brassica napus.BMC plant biology · 2022Article
- Genome-wide identification and characterization of NPF family reveals NtNPF6.13 involving in salt stress inFrontiers in plant science · 2022Article
- Genome-Wide Analysis of Multidrug and Toxic Compound Extruction Transporters in Grape.Frontiers in plant science · 2022Article
- The Expression Characteristics of NPF Genes and Their Response to Vernalization and Nitrogen Deficiency in Rapeseed.International journal of molecular sciences · 2021Article
- Plant Secondary Metabolite Transporters: Diversity, Functionality, and Their Modulation.Frontiers in plant science · 2021Review
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10 authors.
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Abstract
backgroundNITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER (NRT1/PTR) family (NPF) members are essential transporters for many substrates in plants, including nitrate, hormones, peptides, and secondary metabolites. Here, we report the global characterization of NPF in the important oil crop Brassica napus, including that for phylogeny, gene/protein structures, duplications, and expression patterns.
resultsA total of 199 B. napus (BnaNPFs) NPF-coding genes were identified. Phylogenetic analyses categorized these genes into 11 subfamilies, including three new ones. Sequence feature analysis revealed that members of each subfamily contain conserved gene and protein structures. Many hormone-/abiotic stress-responsive cis-acting elements and transcription factor binding sites were identified in BnaNPF promoter regions. Chromosome distribution analysis indicated that BnaNPFs within a subfamily tend to cluster on one chromosome. Syntenic relationship analysis showed that allotetraploid creation by its ancestors (Brassica rapa and Brassica oleracea) (57.89%) and small-scale duplication events (39.85%) contributed to rapid BnaNPF expansion in B. napus. A genome-wide spatiotemporal expression survey showed that NPF genes of each Arabidopsis and B. napus subfamily have preferential expression patterns across developmental stages, most of them are expressed in a few organs. RNA-seq analysis showed that many BnaNPFs (32.66%) have wide exogenous hormone-inductive profiles, suggesting important hormone-mediated patterns in diverse bioprocesses. Homologs in a clade or branch within a given subfamily have conserved organ/spatiotemporal and hormone-inductive profiles, indicating functional conservation during evolution. qRT-PCR-based comparative expression analysis of the 12 BnaNPFs in the NPF2-1 subfamily between high- and low-glucosinolate (GLS) content B. napus varieties revealed that homologs of AtNPF2.9 (BnaNPF2.12, BnaNPF2.13, and BnaNPF2.14), AtNPF2.10 (BnaNPF2.19 and BnaNPF2.20), and AtNPF2.11 (BnaNPF2.26 and BnaNPF2.28) might be involved in GLS transport. qRT-PCR further confirmed the hormone-responsive expression profiles of these putative GLS transporter genes.
conclusionWe identified 199 B. napus BnaNPFs; these were divided into 11 subfamilies. Allopolyploidy and small-scale duplication events contributed to the immense expansion of BnaNPFs in B. napus. The BnaNPFs had preferential expression patterns in different tissues/organs and wide hormone-induced expression profiles. Four BnaNPFs in the NPF2-1 subfamily may be involved in GLS transport. Our results provide an abundant gene resource for further functional analysis of BnaNPFs.
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