Evidence map›Paper›PMID 33287451›Full record

ArticleInternational journal of molecular sciences2020

Comparison of Bisulfite Pyrosequencing and Methylation-Specific qPCR for Methylation Assessment.

Loretta De Chiara, Virginia Leiro-Fernandez, Mar Rodríguez-Girondo, Diana Valverde, María Isabel Botana-Rial, Alberto Fernández-Villar

Open access · goldAbstract read
In one paragraph

Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
0.7field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 19 citations in OpenAlex.

  1. Review
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  8. Exploring Potential Epigenetic Biomarkers for Colorectal Cancer Metastasis.International journal of molecular sciences · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 2 countries.

Loretta De ChiaraDepartment of Biochemistry, Genetics and Immunology, University of Vigo, 36310 Vigo, Spain.ORCID 0000-0001-7700-6417
Virginia Leiro-FernandezPulmonary Department, Hospital Álvaro Cunqueiro, EOXI Vigo, 36213 Vigo, Spain.ORCID 0000-0001-9613-430X
Mar Rodríguez-GirondoDepartment of Medical Statistics and Bioinformatics, Leiden University Medical Center, 2300RC Leiden, The Netherlands.
Diana ValverdeDepartment of Biochemistry, Genetics and Immunology, University of Vigo, 36310 Vigo, Spain.
María Isabel Botana-RialPulmonary Department, Hospital Álvaro Cunqueiro, EOXI Vigo, 36213 Vigo, Spain.
Alberto Fernández-VillarPulmonary Department, Hospital Álvaro Cunqueiro, EOXI Vigo, 36213 Vigo, Spain.
University Hospital Complex Of Vigo · ESUniversidade de Vigo · ESLeiden University Medical Center · NL

Funding

Instituto de Salud Carlos III PI09/90385Secretaria Xeral de Investigación e Desenvolvemento, Xunta de Galicia 09CSA053905PR
6 · The paper itself

Abstract

Different methodological approaches are available to assess DNA methylation biomarkers. In this study, we evaluated two sodium bisulfite conversion-dependent methods, namely pyrosequencing and methylation-specific qPCR (MS-qPCR), with the aim of measuring the closeness of agreement of methylation values between these two methods and its effect when setting a cut-off. Methylation of tumor suppressor gene

Indexed as

DNA MethylationEpigenomicsReal-Time Polymerase Chain ReactionSequence Analysis, DNACyclin-Dependent Kinase Inhibitor p16FemaleHumansMaleROC CurveCyclin-Dependent Kinase Inhibitor p16bisulfite pyrosequencingcut-offcytological lymph node samplesDNA methylationmethylation biomarkermethylation-specific qPCRnon-small cell lung cancer

Identifiers

PMID33287451
PMCPMC7730915
OpenAlexW3108144287

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.