ArticleBiosensors2020
An Improved HRPE-Based Transcriptional Output Reporter to Detect Hypoxia and Anoxia in Plant Tissue.
Article in Biosensors, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 23 citations in OpenAlex.
- The hypoxic niche enclosing the shoot apical meristem is shaped by a combination of morphological features and metabolic activity.Molecular plant · 2026Article
- HNature · 2026Article
- A synthetic ERFVII-dependent circuit in yeast sheds light on the regulation of early hypoxic responses of plants.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Hypoxia-activated fluorescent probes as markers of oxygen levels in plant cells and tissues.The New phytologist · 2025Article
- The HELP-UnaG Fusion Protein as a Bilirubin Biosensor: From Theory to Mature Technological Development.Molecules (Basel, Switzerland) · 2025Review
- The transcription factor ORA59 represses hypoxia responses during Botrytis cinerea infection and reoxygenation.Plant physiology · 2024Article
- Overcoming constraints to measuring O2 diffusivity and consumption of intact roots.Plant physiology · 2024Article
- Recent progress in understanding the cellular and genetic basis of plant responses to low oxygen holds promise for developing flood-resilient crops.Journal of experimental botany · 2024Article
- Genetically Encoded Reporters to Monitor Hypoxia.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Genetically Encoded Biosensors for the Fluorescence Detection of OSensors (Basel, Switzerland) · 2023Review
- Target of rapamycin signaling couples energy to oxygen sensing to modulate hypoxic gene expression inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Acquisition of hypoxia inducibility by oxygen sensing N-terminal cysteine oxidase in spermatophytes.Plant, cell & environment · 2023Article
- Measuring ROS and redox markers in plant cells.RSC chemical biology · 2021Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Oxygen levels in plant tissues may vary, depending on metabolism, diffusion barriers, and environmental availability. Current techniques to assess the oxic status of plant cells rely primarily on invasive microoptodes or Clark-type electrodes, which are not optimally suited for experiments that require high spatial and temporal resolution. In this case, a genetically encoded oxygen biosensor is required instead. This article reports the design, test, and optimization of a hypoxia-signaling reporter, based on five-time repeated hypoxia-responsive promoter elements (HRPE) driving the expression of different reporter proteins. Specifically, this study aimed to improve its performance as a reporter of hypoxic conditions by testing the effect of different untranslated regions (UTRs) at the 5' end of the reporter coding sequence. Next, we characterized an optimized version of the
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.