Evidence map›Paper›PMID 33285040›Full record

ArticleCurrent protocols in microbiology2020

Vibrio parahaemolyticus: Basic Techniques for Growth, Genetic Manipulation, and Analysis of Virulence Factors.

Suneeta Chimalapati, Alexander E Lafrance, Luming Chen, Kim Orth

Abstract read
In one paragraph

Article in Current protocols in microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Article
  2. A critical role forProceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Comparative Metabolomics and Proteomics RevealFrontiers in immunology · 2021
    Article
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Suneeta ChimalapatiDepartment of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas.
Alexander E LafranceDepartment of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas.
Luming ChenDepartment of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas.
Kim OrthDepartment of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas.

Funding

TRAINING THE MD PHD IN THE HEALTH SCIENCE CENTERT32GM008014 · NIGMS · UT SOUTHWESTERN MEDICAL CENTER · PI ZINN, ANDREW R. · 1985 to 2024
$21.1M
MOLECULAR MICROBIOLOGY TRAINING GRANTT32AI007520 · NIAID · UT SOUTHWESTERN MEDICAL CENTER · PI Julie K Pfeiffer · 1997 to 2026
$7.0M
Biochemistry, biology and diversity of Fic domainsR35GM134945 · NIGMS · UT SOUTHWESTERN MEDICAL CENTER · PI ORTH, KIM · 2020 to 2024
$1.8M
Proteostasis, AMPylation and the Unfolded Protein repsonse (UPR)R01GM115188 · NIGMS · UT SOUTHWESTERN MEDICAL CENTER · PI ORTH, KIM · 2015 to 2018
$1.2M
NIAID NIH HHS T32 AI007520NIGMS NIH HHS R01 GM115188NIGMS NIH HHS R35 GM134945NIGMS NIH HHS T32 GM008014
6 · The paper itself

Abstract

Vibrio parahaemolyticus is a Gram-negative, halophilic bacterium and opportunistic pathogen of humans and shrimp. Investigating the mechanisms of V. parahaemolyticus infection and the multifarious virulence factors it employs requires procedures for bacterial culture, genetic manipulation, and analysis of virulence phenotypes. Detailed protocols for growth assessment, generation of mutants, and phenotype assessment are included in this article. © 2020 Wiley Periodicals LLC. Basic Protocol 1: Assessment of growth of V. parahaemolyticus Alternate Protocol 1: Assessment of growth of V. parahaemolyticus using a plate reader Basic Protocol 2: Swimming/swarming motility assay Basic Protocol 3: Genetic manipulation Alternate Protocol 2: Natural transformation Basic Protocol 4: Secretion assay and sample preparation for mass spectrometry analysis Basic Protocol 5: Invasion assay (gentamicin protection assay) Basic Protocol 6: Immunofluorescence detection of intracellular V. parahaemolyticus Basic Protocol 7: Cytotoxicity assay for T3SS2.

Indexed as

Bacterial ProteinsBacteriological TechniquesGentamicinsHeLa CellsHumansStaining and LabelingSwimmingVibrio InfectionsVibrio parahaemolyticusVirulenceVirulence FactorsBacterial ProteinsGentamicinsVirulence Factorscytotoxicitygenetic manipulationgrowthimmunofluorescenceinvasionmotilitysecretion

Identifiers

PMID33285040
PMCPMC7727304

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.