ArticleFrontiers in chemistry2020
Subcellular Singlet Oxygen and Cell Death: Location Matters.
Article in Frontiers in chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 32 citations in OpenAlex.
- A Genetically Encoded Marker for Imaging of Internal Plasmodium Membranes.Molecular microbiology · 2026Article
- Genetically targeted photocatalytic organic dyes for spatiotemporally controlled organic synthesis in specific living cells.Nature chemistry · 2026Article
- Ferroptosis with Contributions from Apoptosis and Necroptosis in Porphyrazine III-Based Photodynamic Therapy of Primary Human Gliomas.Pharmaceutics · 2026Article
- Photodynamic therapy remodels cutaneous tumor immunity: innate priming, suppressive feedback, and translational opportunities.Frontiers in immunology · 2026Review
- Genetic approaches for targeted oxidative stress.NAR cancer · 2025Review
- Article
- Viscous methylene blue formulation for antimicrobial photodynamic therapy in dentistry.Scientific reports · 2025Article
- Generation of singlet oxygen inside living cells: correlation between phosphorescence decay lifetime, localization and outcome of photodynamic action.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2024Article
- Enhanced sonodynamic therapy by carbon dots-shelled microbubbles with focused ultrasound.Ultrasonics sonochemistry · 2023Article
- Significance of Singlet Oxygen Molecule in Pathologies.International journal of molecular sciences · 2023Review
- pH-responsive shell-sheddable polymeric nanoparticles as the photodynamic drug carrier: synthesis, characterization, and in vitro studies.Turkish journal of biology = Turk biyoloji dergisi · 2023Article
- Luminescent Metal Complexes as Emerging Tools for Lipid Imaging.Topics in current chemistry (Cham) · 2022Review
- Antiproliferative and Cytotoxic Activities of Fluorescein-A Diagnostic Angiography Dye.International journal of molecular sciences · 2022Article
- Targeted Formation of 8-Oxoguanine in Telomeres.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Plastid and cytoplasmic origins ofFrontiers in plant science · 2022Article
- Machine Learning Assisted Classification of Cell Lines and Cell States on Quantitative Phase Images.Cells · 2021Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
We developed a tool for targeted generation of singlet oxygen using light activation of a genetically encoded fluorogen-activating protein complexed with a unique dye molecule that becomes a potent photosensitizer upon interaction with the protein. By targeting the protein receptor to activate this dye in distinct subcellular locations at consistent per-cell concentrations, we investigated the impact of localized production of singlet oxygen on induction of cell death. We analyzed light dose-dependent cytotoxic response and characterized the apoptotic vs. necrotic cell death as a function of subcellular location, including the nucleus, the cytosol, the endoplasmic reticulum, the mitochondria, and the membrane. We find that different subcellular origins of singlet oxygen have different potencies in cytotoxic response and the pathways of cell death, and we observed that CT26 and HEK293 cell lines are differentially sensitive to mitochondrially localized singlet oxygen stresses. This work provides new insight into the function of type II reactive oxygen generating photosensitizing processes in inducing targeted cell death and raises interesting mechanistic questions about tolerance and survival mechanisms in studies of oxidative stress in clonal cell populations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.