Evidence map›Paper›PMID 33281753›Full record

ReviewFrontiers in microbiology2020

A Review of Methods to Determine Viability, Vitality, and Metabolic Rates in Microbiology.

Olivier Braissant, Monika Astasov-Frauenhoffer, Tuomas Waltimo, Gernot Bonkat

Abstract readReview
In one paragraph

Review in Frontiers in microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.

0numbers the graph read from it
0cells of the map it votes in
81citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

81 citing papers in PubMed.

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  16. Guidance on biomaterials for periodontal tissue regeneration: Fabrication methods, materials and biological considerations.Dental materials : official publication of the Academy of Dental Materials · 2025
    Review
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  18. Article
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21 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Olivier BraissantDepartment of Biomedical Engineering, Faculty of Medicine, University of Basel, Allschwil, Switzerland.
Monika Astasov-FrauenhofferDepartment Research, University Center for Dental Medicine, University of Basel, Basel, Switzerland.
Tuomas WaltimoDepartment Research, University Center for Dental Medicine, University of Basel, Basel, Switzerland.
Gernot BonkatAlta-Uro AG, Basel, Switzerland.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Viability and metabolic assays are commonly used as proxies to assess the overall metabolism of microorganisms. The variety of these assays combined with little information provided by some assay kits or online protocols often leads to mistakes or poor interpretation of the results. In addition, the use of some of these assays is restricted to simple systems (mostly pure cultures), and care must be taken in their application to environmental samples. In this review, the necessary data are compiled to understand the reactions or measurements performed in many of the assays commonly used in various aspects of microbiology. Also, their relationships to each other, as metabolism links many of these assays, resulting in correlations between measured values and parameters, are discussed. Finally, the limitations of these assays are discussed.

Indexed as

assaycarbon sourceselectron acceptorsmetabolismredox dyesviability

Identifiers

PMID33281753
PMCPMC7705206

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.