ArticleFrontiers in neuroanatomy2020
GluN2B but Not GluN2A for Basal Dendritic Growth of Cortical Pyramidal Neurons.
Article in Frontiers in neuroanatomy, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 22 citations in OpenAlex.
- Neocortical pyramidal cells with axon-carrying basal dendrites: a prominent feature regulated by activity.Brain structure & function · 2026Article
- Mice carrying a GluN2B protein-truncating variant have altered NMDA receptor subunit composition and their behavior recapitulates patient phenotypes.Cellular and molecular life sciences : CMLS · 2026Article
- Perinatal mAChR-mediated activation of cortical subplate neurons elicits network activity driving basket cell differentiation.Frontiers in cellular neuroscience · 2026Article
- Activation of GluN2D-containing NMDA receptors promotes development of axons and axon-carrying dendrites of cortical interneurons.Cerebral cortex (New York, N.Y. : 1991) · 2025Article
- Expression of synaptic proteins and development of dendritic spines in fetal and postnatal neocortex of the pig, the European wild boar Sus scrofa.Brain structure & function · 2025Article
- Chemogenetic activation of Gq signaling modulates dendritic development of cortical neurons in a time- and layer-specific manner.Frontiers in cellular neuroscience · 2025Article
- Reelin differentially shapes dendrite morphology of medial entorhinal cortical ocean and island cells.Development (Cambridge, England) · 2024Article
- Rescuing tri-heteromeric NMDA receptor function: the potential of pregnenolone-sulfate in loss-of-function GRIN2B variants.Cellular and molecular life sciences : CMLS · 2024Article
- Dock4 contributes to neuropathic pain by regulating spinal synaptic plasticity in mice.Frontiers in molecular neuroscience · 2024Article
- Axons of cortical basket cells originating from dendrites develop higher local complexity than axons emerging from basket cell somata.Development (Cambridge, England) · 2023Article
- Optogenetic stimulation shapes dendritic trees of infragranular cortical pyramidal cells.Frontiers in cellular neuroscience · 2023Article
- Extracellular molecular signals shaping dendrite architecture during brain development.Frontiers in cell and developmental biology · 2023Review
- Excitatory Synaptic Transmission in Ischemic Stroke: A New Outlet for Classical Neuroprotective Strategies.International journal of molecular sciences · 2022Review
- Chemogenetic Silencing of Differentiating Cortical Neurons Impairs Dendritic and Axonal Growth.Frontiers in cellular neuroscience · 2022Article
- Distinct regulation of tonic GABAergic inhibition by NMDA receptor subtypes.Cell reports · 2021Article
- Understanding ethanol's acute effects on medial prefrontal cortex neural activity using state-space approaches.Neuropharmacology · 2021Review
- Reelin restricts dendritic growth of interneurons in the neocortex.Development (Cambridge, England) · 2021Article
- An Autism-AssociatedFrontiers in cellular neuroscience · 2021Article
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Authors and funding
14 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
NMDA receptors are important players for neuronal differentiation. We previously reported that antagonizing NMDA receptors with APV blocked the growth-promoting effects evoked by the overexpression of specific calcium-permeable or flip-spliced AMPA receptor subunits and of type I transmembrane AMPA receptor regulatory proteins which both exclusively modify apical dendritic length and branching of cortical pyramidal neurons. These findings led us to characterize the role of GluN2B and GluN2A for dendritogenesis using organotypic cultures of rat visual cortex. Antagonizing GluN2B with ifenprodil and Ro25-6981 strongly impaired basal dendritic growth of supra- and infragranular pyramidal cells at DIV 5-10, but no longer at DIV 15-20. Growth recovered after washout, and protein blots revealed an increase of synaptic GluN2B-containing receptors as indicated by a enhanced phosphorylation of the tyrosine 1472 residue. Antagonizing GluN2A with TCN201 and NVP-AAM077 was ineffective at both ages. Dendrite growth of non-pyramidal interneurons was not altered. We attempted to overexpress GluN2A and GluN2B. However, although the constructs delivered currents in HEK cells, there were neither effects on dendrite morphology nor an enhanced sensitivity to NMDA. Further, co-expressing GluN1-1a and GluN2B did not alter dendritic growth. Visualization of overexpressed, tagged GluN2 proteins was successful after immunofluorescence for the tag which delivered rather weak staining in HEK cells as well as in neurons. This suggested that the level of overexpression is too weak to modify dendrite growth. In summary, endogenous GluN2B, but not GluN2A is important for pyramidal cell basal dendritic growth during an early postnatal time window.
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