ArticleInternational journal of nanomedicine2020
The Effects of Gold Nanoparticles on Leydig Cells and Male Reproductive Function in Mice.
Article in International journal of nanomedicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 58 citations in OpenAlex.
- Assessment of Gold-Mediated Male Reproductive Dysfunction in Male WISTAR Rats Through Sperm Analysis and Testosterone Measurement.Journal of applied toxicology : JAT · 2026Article
- Article
- Benzalkonium Chloride-Coated Iron Oxide Nanoparticles: Cytotoxic Potential on Murine Leydig Cells (TM3).ACS omega · 2025Article
- The dual effects of nanomaterials on sperm and seminal fluid oxidative stress.Materials today. Bio · 2025Review
- Unveiling the nexus between environmental exposures and testicular damages: revelations from autophagy and oxidative stress imbalance.Cell death discovery · 2025Review
- Reproductive Toxicity of Nanoparticles: A Comprehensive Review.Current medicinal chemistry · 2025Review
- Advances in Nanomedicine: Transforming Diagnostic Imaging with Novel Contrast Agents.Current drug metabolism · 2025Review
- The Gut Microbiota Contributes to the Development of LPS-Induced Orchitis by Disrupting the Blood-Testosterone Barrier in Mice.Reproductive sciences (Thousand Oaks, Calif.) · 2024Article
- Titanium Dioxide Nanoparticles Induce Cell Cycle Arrest and Apoptosis through Inhibiting PI3K/AKT/mTOR Pathway in Spermatogonia.Biological trace element research · 2024Article
- NIR-induced photothermal-responsive shape memory polyurethane for versatile smart material applications.RSC advances · 2024Review
- The protective role of phlorizin against lipopolysaccharide-induced acute orchitis in mice associated with changes in gut microbiota composition.Frontiers in veterinary science · 2024Article
- Assessment of reproductive toxicity of gold nanoparticles and its reversibility in male albino rats.Toxicological research · 2024Article
- Nanoparticles-induced potential toxicity on human health: Applications, toxicity mechanisms, and evaluation models.MedComm · 2023Review
- An Overview of Essential Microelements and Common Metallic Nanoparticles and Their Effects on Male Fertility.International journal of environmental research and public health · 2022Review
- Cytotoxicity of Metal-Based Nanoparticles: From Mechanisms and Methods of Evaluation to Pathological Manifestations.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- Melatonin inhibits autophagy in TM3 cells via AKT/FOXO1 pathway.Molecular biology reports · 2022Article
- Biocompatibility and Cytotoxicity of Gold Nanoparticles: Recent Advances in Methodologies and Regulations.International journal of molecular sciences · 2021Review
- Metal Oxide Nanoparticles: Evidence of Adverse Effects on the Male Reproductive System.International journal of molecular sciences · 2021Review
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGold nanoparticles (AuNPs) have shown great promise in various biomedical applications, but their effects on male reproductive function remain to be ascertained. The aim of this study was to investigate the uptake, cytotoxicity and testosterone production inhibition of AuNPs in mouse Leydig cells, as well as their accumulation in the testes of male mice and their effects on male reproductive function.
resultsAuNPs (5 nm) were able to be internalized into the endosomes/lysosomes of TM3 Leydig cells, induce the formation of autophagosomes, increase the production of reactive oxygen species (ROS), and disrupt the cell cycle in S phase, resulting in concentration-dependent cytotoxicity and DNA damage. Interestingly, AuNPs significantly reduced testosterone production in TM3 cells by inhibiting the expression of 17α-hydroxylase, an important enzyme in androgen synthesis. After repeated intravenous injection, AuNPs gradually accumulated and retained in the testes of male BALB/c mice in a dose-dependent manner. One week after withdrawal, the level of plasma testosterone in the 0.5 mg/kg AuNPs group was significantly reduced compared to that in the PBS control group, accompanied by the decreased expression of 17α-hydroxylase in the testes. In addition, AuNPs treatment significantly increased the rate of epididymal sperm malformation, but without affecting fertility.
conclusionOur results suggest that AuNPs can accumulate in the testes and reduce testosterone production in Leydig cells by down-regulating the expression of 17α-hydroxylase, thus affecting the quality of epididymal sperm.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.