ArticleAnalytical sciences : the international journal of the Japan Society for Analytical Chemistry2021
Using Simple-Structured Split Aptamer for Gold Nanoparticle-based Colorimetric Detection of Estradiol.
Article in Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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5 citing papers in PubMed, 27 citations in OpenAlex.
- Review
- [Construction of a 17Se pu = Chinese journal of chromatography · 2025Article
- Turn-off enzyme activity of histidine-rich peptides for the detection of lysozyme.Mikrochimica acta · 2024Article
- Recent research trends in voltammetric sensing platforms for hormones and their applications to human serum analyses.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2022Review
- Recent Advancements in Aptamer-Based Surface Plasmon Resonance Biosensing Strategies.Biosensors · 2021Review
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Authors and funding
2 authors at 1 institution in 1 country.
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Abstract
Demand for the detection of estradiol, which is a naturally occurring hormone, has been increasing. Gold nanoparticle-based colorimetric aptasensors have been developed for estradiol detection; however, the long sequence of aptamers due to the formation of the secondary structure likely affects the sensitivity of the aptasensors. Herein, a sensitive colorimetric biosensor is developed for label-free detection of estradiol by using an estradiol-specific split aptamer. The results demonstrate that a superior response is observed when a split aptamer with a high free energy of the secondary structure (ΔG > -3 kcal/mol) is used, in comparison to that observed using a split aptamer with a low free energy of the secondary structure (ΔG < -3 kcal/mol) at 27°C. After selecting the appropriate split aptamer, the standard calibration curve obtained for estradiol has a detection limit of 6.7 nM, with a linear range of 6.7 nM - 66.7 μM in the logarithmic scale. Furthermore, this assay is sensitive, easy-to-operate, inexpensive, and non-time-consuming (provides results within 50 min), thereby showing potential for clinical applications (detection of other small molecular targets).
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