ArticleVeterinary microbiology2021
SARS-CoV-2 replicates in respiratory ex vivo organ cultures of domestic ruminant species.
Article in Veterinary microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 59 citations in OpenAlex.
- Screening for SARS-CoV-2 in different sheep and goat breeds in Türkiye using in-house ELISAs and real-time PCR.Tropical animal health and production · 2025Article
- Spatial mapping of influenza and coronavirus receptors in the respiratory and intestinal tract epithelium of beef cattle using advanced PixF image analysis.Scientific reports · 2025Article
- Critical amino acid residues in human ACE2 for SARS-CoV-2 spike protein binding and virus entry.Microbiology spectrum · 2025Article
- Investigating SARS-CoV-2 Neutralising Antibody Response in Sheep.Microorganisms · 2024Article
- Cattle, sheep, and goat humoral immune responses against SARS-CoV-2.Veterinary and animal science · 2024Article
- Experimental co-infection of calves with SARS-CoV-2 Delta and Omicron variants of concern.Emerging microbes & infections · 2024Article
- Improving the ONE HEALTH approach: a lesson from SARS-CoV-2 pandemic.Journal of preventive medicine and hygiene · 2024Review
- Serological and Molecular Investigation of SARS-CoV-2 in Horses and Cattle in Switzerland from 2020 to 2022.Viruses · 2024Article
- Susceptibility of bovine to SARS-CoV-2 variants of concern: insights from ACE2, AXL, and NRP1 receptors.Virology journal · 2023Article
- Demonstration of SARS-CoV-2 Exposure in Korean Native Cattle and Korean Native Black Goats in Korea.Animals : an open access journal from MDPI · 2023Article
- First serological evidence of SARS-CoV-2 natural infection in small ruminants : Brief report.Veterinary research communications · 2023Article
- COVID-19 and One Health: potential role of human and animals in SARS-CoV-2 life cycle.Science in One Health · 2023Review
- Susceptibility of sheep to experimental co-infection with the ancestral lineage of SARS-CoV-2 and its alpha variant.Emerging microbes & infections · 2022Article
- SARS CoV-2 infections in animals, two years into the pandemic.Archives of virology · 2022Review
- A Deletion Encompassing the Furin Cleavage Site in the Spike Encoding Gene Does Not Alter SARS-CoV-2 Replication in Lung Tissues of Mink and Neutralization by Convalescent Human Serum Samples.Pathogens (Basel, Switzerland) · 2022Article
- Distribution of Coronavirus Receptors in the Swine Respiratory and Intestinal Tract.Veterinary sciences · 2022Article
- Differential susceptibility of SARS-CoV-2 in animals: Evidence of ACE2 host receptor distribution in companion animals, livestock and wildlife by immunohistochemical characterisation.Transboundary and emerging diseases · 2022Article
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- First Description of Serological Evidence for SARS-CoV-2 in Lactating Cows.Animals : an open access journal from MDPI · 2022Article
- Article
Corrections and comments
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Authors and funding
20 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There is strong evidence that severe acute respiratory syndrome 2 virus (SARS-CoV-2), the causative agent of the coronavirus disease 2019 (COVID-19) pandemic, originated from an animal reservoir. However, the exact mechanisms of emergence, the host species involved, and the risk to domestic and agricultural animals are largely unknown. Some domestic animal species, including cats, ferrets, and minks, have been demonstrated to be susceptible to SARS-CoV-2 infection, while others, such as pigs and chickens, are not. Importantly, the susceptibility of ruminants to SARS-CoV-2 is unknown, even though they often live in close proximity to humans. We investigated the replication and tissue tropism of two different SARS-CoV-2 isolates in the respiratory tract of three farm animal species - cattle, sheep, and pigs - using respiratory ex vivo organ cultures (EVOCs). We demonstrate that the respiratory tissues of cattle and sheep, but not of pigs, sustain viral replication in vitro of both isolates and that SARS-CoV-2 is associated to ACE2-expressing cells of the respiratory tract of both ruminant species. Intriguingly, a SARS-CoV-2 isolate containing an amino acid substitution at site 614 of the spike protein (mutation D614G) replicated at higher magnitude in ex vivo tissues of both ruminant species, supporting previous results obtained using human cells. These results suggest that additional in vivo experiments involving several ruminant species are warranted to determine their potential role in the epidemiology of this virus.
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