Evidence map›Paper›PMID 33277554›Full record

ArticleScientific reports2020

Ascorbic acid during the suckling period is required for proper DNA demethylation in the liver.

Kenichi Kawahori, Yoshitaka Kondo, Xunmei Yuan, Yuki Kawasaki, Nozomi Hanzawa, Kazutaka Tsujimoto, Fumiko Wada, Takashi Kohda, Akihito Ishigami, Tetsuya Yamada and 2 more

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
0.9field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 19 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Review
  5. TargetingCancers · 2022
    Review
  6. Ascorbate Is a Primary Antioxidant in Mammals.Molecules (Basel, Switzerland) · 2022
    Review
  7. The Epigenetic Role of Vitamin C in Neurodevelopment.International journal of molecular sciences · 2022
    Review
  8. Review
  9. Review
  10. Microbial Cell Factories for Green Production of Vitamins.Frontiers in bioengineering and biotechnology · 2021
    Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 1 country.

Kenichi KawahoriDepartment of Molecular Endocrinology and Metabolism, Graduate School of Medical and Dental Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, 113-8510, Japan.
Yoshitaka KondoMolecular Regulation of Aging, Tokyo Metropolitan Institute of Gerontology, Tokyo, 173-0015, Japan.
Xunmei YuanDepartment of Molecular Endocrinology and Metabolism, Graduate School of Medical and Dental Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, 113-8510, Japan.
Yuki KawasakiDepartment of Epigenetics, Medical Research Institute, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, 113-8510, Japan.
Nozomi HanzawaDepartment of Molecular Endocrinology and Metabolism, Graduate School of Medical and Dental Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, 113-8510, Japan.
Kazutaka TsujimotoDepartment of Molecular Endocrinology and Metabolism, Graduate School of Medical and Dental Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, 113-8510, Japan.
Fumiko WadaDepartment of Molecular Endocrinology and Metabolism, Graduate School of Medical and Dental Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, 113-8510, Japan.
Takashi KohdaDepartment of Epigenetics, Medical Research Institute, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, 113-8510, Japan.
Akihito IshigamiMolecular Regulation of Aging, Tokyo Metropolitan Institute of Gerontology, Tokyo, 173-0015, Japan.
Tetsuya YamadaDepartment of Molecular Endocrinology and Metabolism, Graduate School of Medical and Dental Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, 113-8510, Japan.
Yoshihiro OgawaDepartment of Molecular and Cellular Metabolism, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, 113-8510, Japan. yogawa@med.kyushu-u.ac.jp.
Koshi HashimotoDepartment of Preemptive Medicine and Metabolism, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510, Japan. k-hashi@dokkyomed.ac.jp.
Tokyo Medical and Dental University · JPTokyo Metropolitan Institute of Gerontology · JPWaseda University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Ascorbic acid (AA, vitamin C) serves as a cofactor for ten-eleven translocation (TET) enzymes and induces DNA demethylation in vitro. However, its role in DNA demethylation in vivo remains unclear. We previously reported that DNA demethylation in the mouse liver was enhanced during the suckling period. Therefore, we hypothesized that DNA demethylation is enhanced in an AA-dependent manner during the suckling period. To examine our hypothesis, we employed wild-type (WT) mice, which synthesize AA, and senescence marker protein-30/gluconolactonase (SMP30/GNL) knockout (KO) mice, which cannot synthesize AA, and analyzed the DNA methylation status in the livers of offspring in both the suckling period and adulthood. SMP30/GNL KO offspring showed DNA hypermethylation in the liver possibly due to low plasma and hepatic AA levels during the suckling period despite the administration of rescue-dose AA to dams. Furthermore, DNA hypermethylation of the fibroblast growth factor 21 gene (Fgf21), a PPARα target gene, persisted into adulthood. In contrast, a high-dose AA administration to SMP30/GNL KO dams during the lactation period restored DNA demethylation in the livers of offspring. Even though a slight increase was observed in plasma AA levels with the administration of rescue-dose AA to WT dams during the gestation and lactation periods, DNA demethylation in the livers of offspring was minimally enhanced. The present results demonstrate that AA intake during the suckling period is required for proper DNA demethylation in the liver.

Indexed as

DNA DemethylationAnimalsAnimals, SucklingAscorbic AcidCalcium-Binding ProteinsCarboxylic Ester HydrolasesEmbryo, MammalianFatty AcidsFemaleFibroblast Growth FactorsGene Expression Regulation, DevelopmentalHigh-Throughput Nucleotide SequencingIntracellular Signaling Peptides and ProteinsLactationLipid MetabolismLiverAscorbic AcidCalcium-Binding ProteinsCarboxylic Ester HydrolasesFatty Acidsfibroblast growth factor 21Fibroblast Growth FactorsgluconolactonaseIntracellular Signaling Peptides and ProteinsPPAR alphaRgn protein, mouse

Identifiers

PMID33277554
PMCPMC7718234
OpenAlexW3108898660

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.