ArticleBiochemical and biophysical research communications2021
Dual ELISA using SARS-CoV-2 nucleocapsid protein produced in E. coli and CHO cells reveals epitope masking by N-glycosylation.
Article in Biochemical and biophysical research communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 27 citations in OpenAlex.
- Precise mapping of two conserved minimal epitopes on the nucleocapsid protein of porcine epidemic diarrhea virus.Frontiers in microbiology · 2026Article
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- Conducting polymer functionalized Cu-metal organic framework-based electrochemical immunosensor for rapid and sensitive quantitation of Escherichia coli O157:H7.Mikrochimica acta · 2024Article
- The Key to Increase Immunogenicity of Next-Generation COVID-19 Vaccines Lies in the Inclusion of the SARS-CoV-2 Nucleocapsid Protein.Journal of immunology research · 2024Review
- Revisiting the interaction between complement lectin pathway protease MASP-2 and SARS-CoV-2 nucleoprotein.Frontiers in immunology · 2024Article
- Transient Expression in HEK-293 Cells in Suspension Culture as a Rapid and Powerful Tool: SARS-CoV-2 N and Chimeric SARS-CoV-2N-CD154 Proteins as a Case Study.Biomedicines · 2023Article
- The SARS-CoV-2 nucleocapsid protein: its role in the viral life cycle, structure and functions, and use as a potential target in the development of vaccines and diagnostics.Virology journal · 2023Review
- Production and Characterization of a SARS-CoV-2 Nucleocapsid Protein Reference Material.ACS measurement science au · 2022Article
- A novel photoelectrochemical immunosensor based on TiOMicrochemical journal : devoted to the application of microtechniques in all branches of science · 2022Article
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- Molecularly Imprinted Polymer-Based Sensors for SARS-CoV-2: Where Are We Now?Biomimetics (Basel, Switzerland) · 2022Review
- Molecularly imprinted polymer based electrochemical sensor for quantitative detection of SARS-CoV-2 spike protein.Sensors and actuators. B, Chemical · 2022Article
- Graphene-Based Femtogram-Level Sensitive Molecularly Imprinted Polymer of SARS-CoV-2.Advanced materials interfaces · 2021Article
- Glycosylation of SARS-CoV-2: structural and functional insights.Analytical and bioanalytical chemistry · 2021Review
- Variable posttranslational modifications of severe acute respiratory syndrome coronavirus 2 nucleocapsid protein.Glycobiology · 2021Article
- A comprehensive antigen production and characterisation study for easy-to-implement, specific and quantitative SARS-CoV-2 serotests.EBioMedicine · 2021Article
- Development of a portable MIP-based electrochemical sensor for detection of SARS-CoV-2 antigen.Biosensors & bioelectronics · 2021Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Spike and nucleocapsid proteins of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2-SP, SARS-CoV-2-NP) are the main immunogenic targets for antibodies. We herein demonstrate that the glycosylation of SARS-CoV-2-NP masks some of its antibody epitopes. In many cases, this can lead to false-negative serological tests. Deglycosylation of SARS-CoV-2-NP significantly increased the number of positive tests. The glycosylation pattern analysis of this protein revealed that the putative N-linked glycosylation sites, at the amino acid positions 48 and 270, co-located with two of the main immunodominant B cell epitopes.
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