Evidence map›Paper›PMID 33276600›Full record

ArticleViruses2020

An Update of the Virion Proteome of Kaposi Sarcoma-Associated Herpesvirus.

Ramina Nabiee, Basir Syed, Jesus Ramirez Castano, Rukhsana Lalani, Jennifer E Totonchy

Open access · goldAbstract read
In one paragraph

Article in Viruses, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
1.0field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 21 citations in OpenAlex.

  1. Review
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  6. Comparative Review of the Conserved UL24 Protein Family in Herpesviruses.International journal of molecular sciences · 2024
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Ramina NabieeBiomedical and Pharmaceutical Sciences Department, Chapman University School of Pharmacy, Irvine, CA 92618, USA.ORCID 0000-0001-9806-8481
Basir SyedBiomedical and Pharmaceutical Sciences Department, Chapman University School of Pharmacy, Irvine, CA 92618, USA.ORCID 0000-0002-3407-9525
Jesus Ramirez CastanoBiomedical and Pharmaceutical Sciences Department, Chapman University School of Pharmacy, Irvine, CA 92618, USA.
Rukhsana LalaniBiomedical and Pharmaceutical Sciences Department, Chapman University School of Pharmacy, Irvine, CA 92618, USA.
Jennifer E TotonchyBiomedical and Pharmaceutical Sciences Department, Chapman University School of Pharmacy, Irvine, CA 92618, USA.ORCID 0000-0002-8444-8536
Chapman University · US

Funding

Factors Influencing KSHV Infection and Spread within the Oral CavityR00DE024969 · NIDCR · CHAPMAN UNIVERSITY · PI TOTONCHY, JENNIFER E · 2017 to 2019
$747k
NIDCR NIH HHS R00 DE024969
6 · The paper itself

Abstract

The virion proteins of Kaposi sarcoma-associated herpesvirus (KSHV) were initially characterized in 2005 in two separate studies that combined the detection of 24 viral proteins and a few cellular components via LC-MS/MS or MALDI-TOF. Despite considerable advances in the sensitivity and specificity of mass spectrometry instrumentation in recent years, leading to significantly higher yields in detections, the KSHV virion proteome has not been revisited. In this study, we have re-examined the protein composition of purified KSHV virions via ultra-high resolution Qq time-of-flight mass spectrometry (UHR-QqTOF). Our results confirm the detection of all previously reported virion proteins, in addition to 17 other viral proteins, some of which have been characterized as virion-associated using other methods, and 10 novel proteins identified as virion-associated for the first time in this study. These results add KSHV ORF9, ORF23, ORF35, ORF48, ORF58, ORF72/vCyclin, K3, K9/vIRF1, K10/vIRF4, and K10.5/vIRF3 to the list of KSHV proteins that can be incorporated into virions. The addition of these proteins to the KSHV virion proteome provides novel and important insight into early events in KSHV infection mediated by virion-associated proteins. Data are available via ProteomeXchange with identifier PXD022626.

Indexed as

ProteomeProteomicsCell LineChemical FractionationChromatography, LiquidHerpesviridae InfectionsHerpesvirus 8, HumanHumansProtein Processing, Post-TranslationalQuality ControlTandem Mass SpectrometryViral ProteinsVirionProteomeViral Proteinsbottom-up shotgun proteomicsKSHV virion proteomeUHR-QqTOFviral proteomics

Identifiers

PMID33276600
PMCPMC7761624
OpenAlexW3108226843

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.