DNA alternate polymerase PolB mediates inhibition of type III secretion in Pseudomonas aeruginosa.

Shubham Chakravarty, Layla Ramos-Hegazy, Abigail Gasparovic, Gregory G Anderson

Open access · greenAbstract read

In one paragraph

Article in Microbes and infection. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

0.2field-weighted citation impact, top 49% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

Targeting Essential Hypothetical Proteins ofBioMed research international · 2023
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Shubham ChakravartyDepartment of Biology, Indiana University Purdue University Indianapolis, Indianapolis, IN, USA.

Layla Ramos-HegazyDepartment of Biology, Indiana University Purdue University Indianapolis, Indianapolis, IN, USA.

Abigail GasparovicDepartment of Biology, Indiana University Purdue University Indianapolis, Indianapolis, IN, USA.

Gregory G AndersonDepartment of Biology, Indiana University Purdue University Indianapolis, Indianapolis, IN, USA. Electronic address: ga2@iupui.edu.

University of Indianapolis · US

Funding

A novel bacterial protease and its involvement in wound infectionR03AR068724 · NIAMS · INDIANA UNIVERSITY INDIANAPOLIS · PI ANDERSON, GREGORY G, KUSMIERCZYK, ANDREW R · 2015 to 2017

$229k

NIAMS NIH HHS R03 AR068724

6 · The paper itself

Abstract

Opportunistic pathogen Pseudomonas aeruginosa uses a variety of virulence factors to cause acute and chronic infections. We previously found that alternate DNA polymerase gene polB inhibits P. aeruginosa pyocyanin production. We investigated whether polB also affects T3SS expression. polB overexpression significantly reduced T3SS transcription and repressed translation of the master T3SS regulator ExsA, while not affecting exsA mRNA transcript abundance. Further, polB does not act through previously described genetic pathways that post-transcriptionally regulate ExsA. Our results show a novel T3SS regulatory component which may lead to development of future drugs to target this mechanism.

Indexed as

Bacterial ProteinsDNA Polymerase betaGene Expression Regulation, BacterialPseudomonas aeruginosaTranscription, GeneticType III Secretion SystemsBacterial ProteinsDNA Polymerase betaType III Secretion SystemsPseudomonas aeruginosaStressType III secretion

Identifiers

PMID33276123

DOI10.1016/j.micinf.2020.11.004

PMCPMC8084926

OpenAlexW3108540965

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.