ArticleeLife2020
Ultrastructural comparison of dendritic spine morphology preserved with cryo and chemical fixation.
Article in eLife, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed.
- Spine-neck electrical bottlenecks tune temporal precision and inhibitory gating in cortical pyramidal neurons: A connectomics-based biophysical study.bioRxiv : the preprint server for biology · 2026Article
- Memory engram synapse 3D macromolecular architecture visualized by cryoCLEM-guided cryoET.Structure (London, England : 1993) · 2026Article
- Volume electron microscopy in axon regeneration research: insights into mitochondria, endoplasmic reticulum, and membrane contacts.Frontiers in neural circuits · 2026Review
- Comparative study of pre- and post-mortem perfusion of fixative for the quality of neuronal tissue preparation.Lab animal · 2025Article
- ER morphological analysis associated with interstitial cells of Cajal and smooth muscle cells in the murine stomach.Cell and tissue research · 2025Article
- Calcium dynamics in small spaces: Lessons learned from modeling in dendritic spines.Biophysical journal · 2025Review
- Correlative light and electron microscopy for human brain and other biological models.Nature protocols · 2025Review
- Immunoelectron microscopy: a comprehensive guide from sample preparation to high-resolution imaging.Discover nano · 2025Review
- VSOT: volume-surface optimization for accurate ultrastructure analysis of dendritic spines.Bioinformatics (Oxford, England) · 2025Article
- Expression of synaptic proteins and development of dendritic spines in fetal and postnatal neocortex of the pig, the European wild boar Sus scrofa.Brain structure & function · 2025Article
- Memory engram synapse 3D molecular architecture visualized by cryoCLEM-guided cryoET.bioRxiv : the preprint server for biology · 2025Article
- What are memories made of? A survey of neuroscientists on the structural basis of long-term memory.PloS one · 2025Article
- Membrane mechanics dictate axonal pearls-on-a-string morphology and function.Nature neuroscience · 2025Article
- Native Cryo-Correlative Light and Synchrotron X-ray Fluorescence Imaging of Proteins and Essential Metals in Subcellular Neuronal Compartments.Chemical & biomedical imaging · 2024Article
- Dynamic changes in endoplasmic reticulum morphology and its contact with the plasma membrane in motor neurons in response to nerve injury.Cell and tissue research · 2024Article
- Fluorescence microscopy shadow imaging for neuroscience.Frontiers in cellular neuroscience · 2024Review
- Information encoded in volumes and areas of dendritic spines is nearly maximal across mammalian brains.Scientific reports · 2023Article
- Rehydration of Freeze Substituted Brain Tissue for Pre-embedding Immunoelectron Microscopy.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2023Article
- The Impact of Chemical Fixation on the Microanatomy of Mouse Organotypic Hippocampal Slices.eNeuro · 2023Article
- Three-dimensional ultrastructure analysis of organelles in injured motor neuron.Anatomical science international · 2023Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Previously, we showed that cryo fixation of adult mouse brain tissue gave a truer representation of brain ultrastructure in comparison with a standard chemical fixation method (Korogod et al., 2015). Extracellular space matched physiological measurements, there were larger numbers of docked vesicles and less glial coverage of synapses and blood capillaries. Here, using the same preservation approaches, we compared the morphology of dendritic spines. We show that the length of the spine and the volume of its head is unchanged; however, the spine neck width is thinner by more than 30% after cryo fixation. In addition, the weak correlation between spine neck width and head volume seen after chemical fixation was not present in cryo-fixed spines. Our data suggest that spine neck geometry is independent of the spine head volume, with cryo fixation showing enhanced spine head compartmentalization and a higher predicted electrical resistance between spine head and parent dendrite.
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