Evidence map›Paper›PMID 33274277›Full record

ArticleACS central science2020

Bioorthogonal Correlative Light-Electron Microscopy of

Thomas Bakkum, Matthias T Heemskerk, Erik Bos, Mirjam Groenewold, Nikolaos Oikonomeas-Koppasis, Kimberley V Walburg, Suzanne van Veen, Martijn J C van der Lienden, Tyrza van Leeuwen, Marielle C Haks and 3 more

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In one paragraph

Article in ACS central science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Thomas BakkumLeiden Institute of Chemistry and The Institute of Chemical Immunology, Leiden University, Einsteinweg 55, Leiden 2300 RA, The Netherlands.
Matthias T HeemskerkDepartment of Infectious Diseases, Leiden University Medical Center, Albinusdreef 2, 2333 ZC Leiden, The Netherlands.
Erik BosDepartment of Cell and Chemical Biology, Leiden University Medical Center, Einthovenweg 20, 2333 ZC Leiden, The Netherlands.
Mirjam GroenewoldLeiden Institute of Chemistry and The Institute of Chemical Immunology, Leiden University, Einsteinweg 55, Leiden 2300 RA, The Netherlands.
Nikolaos Oikonomeas-KoppasisLeiden Institute of Chemistry and The Institute of Chemical Immunology, Leiden University, Einsteinweg 55, Leiden 2300 RA, The Netherlands.
Kimberley V WalburgDepartment of Infectious Diseases, Leiden University Medical Center, Albinusdreef 2, 2333 ZC Leiden, The Netherlands.
Suzanne van VeenDepartment of Infectious Diseases, Leiden University Medical Center, Albinusdreef 2, 2333 ZC Leiden, The Netherlands.
Martijn J C van der LiendenLeiden Institute of Chemistry and The Institute of Chemical Immunology, Leiden University, Einsteinweg 55, Leiden 2300 RA, The Netherlands.
Tyrza van LeeuwenLeiden Institute of Chemistry and The Institute of Chemical Immunology, Leiden University, Einsteinweg 55, Leiden 2300 RA, The Netherlands.
Marielle C HaksDepartment of Infectious Diseases, Leiden University Medical Center, Albinusdreef 2, 2333 ZC Leiden, The Netherlands.
Tom H M OttenhoffDepartment of Infectious Diseases, Leiden University Medical Center, Albinusdreef 2, 2333 ZC Leiden, The Netherlands.
Abraham J KosterDepartment of Cell and Chemical Biology, Leiden University Medical Center, Einthovenweg 20, 2333 ZC Leiden, The Netherlands.
Sander I van KasterenLeiden Institute of Chemistry and The Institute of Chemical Immunology, Leiden University, Einsteinweg 55, Leiden 2300 RA, The Netherlands.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bioorthogonal correlative light-electron microscopy (B-CLEM) can give a detailed overview of multicomponent biological systems. It can provide information on the ultrastructural context of bioorthogonal handles and other fluorescent signals, as well as information about subcellular organization. We have here applied B-CLEM to the study of the intracellular pathogen

Identifiers

PMID33274277
PMCPMC7706097

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.