ArticleNature biomedical engineering2020
Rapid lateral flow immunoassay for the fluorescence detection of SARS-CoV-2 RNA.
Article in Nature biomedical engineering, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 113 papers, 2 of them syntheses that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
113 citing papers in PubMed, 2 syntheses or guidelines pooled it, 296 citations in OpenAlex.
- Laboratory-based molecular test alternatives to RT-PCR for the diagnosis of SARS-CoV-2 infection.The Cochrane database of systematic reviews · 2024Pooled it
- Rapid, point-of-care antigen tests for diagnosis of SARS-CoV-2 infection.The Cochrane database of systematic reviews · 2022Pooled it
- Analysis of multiplexed SERS-based lateral flow assays: turning qualitative into quantitative for inflammatory bowel disease monitoring.The Analyst · 2026Article
- Efficient fabrication of paper-based microfluidic biosensors incorporating laser-induced graphene for label-free electrochemical immunoassays.Mikrochimica acta · 2026Article
- Approaching Gold-Standard Sensitivity in a Portable and Versatile Gold Nanoprisms Thermoplasmonic Platform for Lateral Flow Detection of Biomolecules.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Three-Dimensional Magnetic Multi-Metallic Nanozymes With Colorimetric and Catalytic Amplification for Rapid and Highly-Sensitive Detection of Influenza A.Exploration (Beijing, China) · 2026Article
- Vertical-flow tearable paper-tape rolls for scalable multiplexed point-of-care nucleic acid testing.Microsystems & nanoengineering · 2026Article
- Recognition, Signal Transduction, and Amplification of Nucleic Acids Under Isothermal Condition.Advanced genetics (Hoboken, N.J.) · 2026Article
- High-sensitivity, protein-independent detection of dsDNA sequences.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- [Research progress on paper-based microfluidic chips in pathogen detection].Se pu = Chinese journal of chromatography · 2025Review
- Development of a Peptide Aptamer-Based TRFIA for the Quantitive Detection of SARS-CoV-2 Nucleocapsid Protein.Journal of fluorescence · 2025Article
- An immunoassay based on polyHRP signal amplification for HPV16/18 E6 RNA detection with anti-DNA-RNA hybrid antibody.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025Article
- An energy-embodied paralleled liquid manipulation for equipment-free, quantitative multiplexed liver function monitoring.Science advances · 2025Article
- Rational Synthesis of Uniform Au Nanospheres under One-Shot Injection: From Mechanistic Understanding to Experimental Control.Precision chemistry · 2025Article
- Plasmonic coffee-ring biosensing for AI-assisted point-of-care diagnostics.Nature communications · 2025Article
- Lateral flow immunoassay using plasmonic scattering.Nature communications · 2025Article
- pH-Responsive Triplex DNA Nanoswitches: Surface Plasmon Resonance Platform for Bladder Cancer-Associated microRNAs.ACS nano · 2025Article
- Implantable photoelectrochemical-therapeutic methotrexate monitoring system with dual-atomic docking strategy.Nature communications · 2025Article
- Rapid diagnosis of different cardiovascular disease events from early released cardiac biomarkers, cTnI, BNP, and CRP, by biosensor technology.Frontiers in cardiovascular medicine · 2025Article
- A facile optical fiber-embedded microfluidic biochip for rapid and sensitive detection of microRNA-let-7a in serum.Mikrochimica acta · 2024Article
53 more citing papers are in PubMed but not listed here.
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Authors and funding
19 authors at 8 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The coronavirus disease 2019 (COVID-19) pandemic has highlighted the need for rapid and accurate nucleic acid detection at the point of care. Here, we report an amplification-free nucleic acid immunoassay, implemented on a lateral flow strip, for the fluorescence detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in less than one hour. The assay uses DNA probes that are designed to bind to the conserved open reading frame 1ab (ORF1ab), envelope protein (E) and the nucleocapsid (N) regions of the SARS-CoV-2 genome, and a fluorescent-nanoparticle-labelled monoclonal antibody that binds to double-stranded DNA-RNA hybrids. In a multi-hospital randomized double-blind trial involving 734 samples (593 throat swabs and 141 sputum) provided by 670 individuals, the assay achieved sensitivities of 100% and specificities of 99% for both types of sample (ground truth was determined using quantitative PCR with reverse transcription). The inexpensive amplification-free detection of SARS-CoV-2 RNA should facilitate the rapid diagnosis of COVID-19 at the point of care.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.