Evidence map›Paper›PMID 33273557›Full record

ArticleScientific reports2020

Improve sample preparation process for miRNA isolation from the culture cells by using silica fiber membrane.

Wen-Pin Hu, Yu-Chi Chen, Wen-Yih Chen

Abstract read
In one paragraph

Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Comprehensive Review on Viral RNA Extraction Strategies for Enhanced Molecular Diagnostics.Interdisciplinary perspectives on infectious diseases · 2025
    Review
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Review
  12. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Wen-Pin HuDepartment of Bioinformatics and Medical Engineering, Asia University, Taichung, 41354, Taiwan.ORCID 0000-0003-1826-7689
Yu-Chi ChenDepartment of Chemical and Materials Engineering, National Central University, Jhong-Li, No. 300, Zhongda Rd., Zhongli District, Taoyuan City, 32001, Taiwan.
Wen-Yih ChenDepartment of Chemical and Materials Engineering, National Central University, Jhong-Li, No. 300, Zhongda Rd., Zhongli District, Taoyuan City, 32001, Taiwan. wychen@ncu.edu.tw.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In clinical applications of miRNAs, the purity and quality of the testing samples are very critical, especially the obtained tissue sample volume is limited. If the extracted miRNAs are contaminated or different in quality before analysis, it will increase the variance of the analysis result and make the medical information judgment incorrect and cannot be portable. Herein, we improved the commercially extraction kit by realizing the fundamental mechanism and hoped to serve finding optimal procedures for increasing the recovery of miRNAs extracted from cultured cells. In the adsorption process, the factors, like increasing the ethanol concentration or adding Ca

Indexed as

Cell Culture TechniquesGene Expression ProfilingHCT116 CellsHumansMicroRNAsSilicon DioxideSpecimen HandlingMicroRNAsSilicon Dioxide

Identifiers

PMID33273557
PMCPMC7713297

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.