ArticleNature communications2020
Pathway engineering in yeast for synthesizing the complex polyketide bikaverin.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 62 citations in OpenAlex.
- Design of an oral vaccine againstThe veterinary quarterly · 2026Article
- Yeast species resource exploration and application in cell factories.Synthetic and systems biotechnology · 2026Review
- Biosynthesis and Microbial Production of Carminic Acid: From Pathway Elucidation to Synthetic Biology.Microorganisms · 2026Review
- High-Level Production of Ergothioneine in Yarrowia lipolytica via Systematic Metabolic Engineering Strategies.Biotechnology and bioengineering · 2026Article
- Theoretical Investigation of Structural and Optical Peculiarities of Bikaverin Fungal Pigment in Chloroform Solution.Molecules (Basel, Switzerland) · 2025Article
- Transcriptomic Response Pathways of Yeast to Crucial Polyphenolic Acids in Rosmarinus Acid Biosynthesis.Current microbiology · 2025Article
- Bikaverin as a molecular weapon: enhancing Fusarium oxysporum pathogenicity in bananas via rhizosphere microbiome manipulation.Microbiome · 2025Article
- Redesigned Pathway forJACS Au · 2025Article
- Efficient de novo production of bioactive cordycepin by Aspergillus oryzae using a food-grade expression platform.Microbial cell factories · 2023Article
- Advances in the dynamic control of metabolic pathways inEngineering microbiology · 2023Review
- Dissecting aneuploidy phenotypes by constructing Sc2.0 chromosome VII and SCRaMbLEing synthetic disomic yeast.Cell genomics · 2023Article
- Optimization of central carbon metabolism by Warburg effect of human cancer cell improves triterpenes biosynthesis in yeast.Advanced biotechnology · 2023Article
- CREEPY: CRISPR-mediated editing of synthetic episomes in yeast.Nucleic acids research · 2023Article
- Rethinking Biosynthesis of Aclacinomycin A.Molecules (Basel, Switzerland) · 2023Review
- Production of Fungal Pigments: Molecular Processes and Their Applications.Journal of fungi (Basel, Switzerland) · 2022Review
- Speed dating for enzymes! Finding the perfect phosphopantetheinyl transferase partner for your polyketide synthase.Microbial cell factories · 2022Article
- Expression of bik cluster and production of bikaverin by Fusarium oxysporum f. sp. lycopersici grown using two alternate nitrogen sources.International microbiology : the official journal of the Spanish Society for Microbiology · 2022Article
- Physiological limitations and opportunities in microbial metabolic engineering.Nature reviews. Microbiology · 2022Review
- Recovery and purification of bikaverin produced byFood chemistry: X · 2021Article
- Saccharomyces cerevisiae as host for the recombinant production of polyketides and nonribosomal peptides.Microbial cell factories · 2021Review
Corrections and comments
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Authors and funding
14 authors at 5 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fungal polyketides display remarkable structural diversity and bioactivity, and therefore the biosynthesis and engineering of this large class of molecules is therapeutically significant. Here, we successfully recode, construct and characterize the biosynthetic pathway of bikaverin, a tetracyclic polyketide with antibiotic, antifungal and anticancer properties, in S. cerevisiae. We use a green fluorescent protein (GFP) mapping strategy to identify the low expression of Bik1 (polyketide synthase) as a major bottleneck step in the pathway, and a promoter exchange strategy is used to increase expression of Bik1 and bikaverin titer. Then, we use an enzyme-fusion strategy to directly couple the monooxygenase (Bik2) and methyltransferase (Bik3) to efficiently channel intermediates between modifying enzymes, leading to an improved titer of bikaverin at 202.75 mg/L with flask fermentation (273-fold higher than the initial titer). This study demonstrates that the biosynthesis of complex fungal polyketides can be established and efficiently engineered in S. cerevisiae, highlighting the potential for natural product synthesis and large-scale fermentation in yeast.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.