Evidence map›Paper›PMID 33272974›Full record

ArticleCold Spring Harbor protocols2021

Live Imaging of Cytoskeletal Dynamics in Embryonic

Burcu Erdogan, Elizabeth A Bearce, Laura Anne Lowery

Open access · greenAbstract read
In one paragraph

Article in Cold Spring Harbor protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Imaging Methods inCold Spring Harbor protocols · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 3 institutions in 1 country.

Burcu Erdogan *Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
Elizabeth A Bearce *Institute of Molecular Biology, Department of Biology, University of Oregon, Eugene, Oregon 97403, USA.
Laura Anne LoweryBoston University School of Medicine, Boston Medical Center, Boston, Massachusetts 02118, USA lalowery@bu.edu.ORCID 0000-0001-8959-2679
Boston University · USHarvard University · USUniversity of Oregon · US

Funding

Elucidating mechanistic connections between guidance signaling, microtubule regulation, and growth cone steering: Diversity SupplementR01MH109651 · NIMH · BOSTON MEDICAL CENTER · PI Laura Anne LOWERY · 2016 to 2026
$4.3M
Role of TACC3 in Cranial Neural Crest Cell Migration:Implications for Craniofacial DisordersR03DE025824 · NIDCR · BOSTON COLLEGE · PI LOWERY, LAURA ANNE · 2016 to 2017
$235k
NIDCR NIH HHS R03 DE025824NIMH NIH HHS R01 MH109651
6 · The paper itself

Abstract

The cytoskeleton is a dynamic, fundamental network that not only provides mechanical strength to maintain a cell's shape but also controls critical events like cell division, polarity, and movement. Thus, how the cytoskeleton is organized and dynamically regulated is critical to our understanding of countless processes. Live imaging of fluorophore-tagged cytoskeletal proteins allows us to monitor the dynamic nature of cytoskeleton components in embryonic cells. Here, we describe a protocol to monitor and analyze cytoskeletal dynamics in primary embryonic neuronal growth cones and neural crest cells obtained from

Indexed as

Actin CytoskeletonAnimalsCells, CulturedCytoskeletonEmbryo, NonmammalianGrowth ConesMicroscopy, ConfocalMicroscopy, FluorescenceMicrotubulesNeural CrestNeuronsXenopus laevis

Identifiers

PMID33272974
PMCPMC8026486
OpenAlexW3108125302

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.