ArticleStem cell research & therapy2020
Synthesis of aligned porous polyethylene glycol/silk fibroin/hydroxyapatite scaffolds for osteoinduction in bone tissue engineering.
Article in Stem cell research & therapy, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 32 citations in OpenAlex.
- Surface-Exposed Hydroxyapatite Microparticles in Electrospun PLLA Scaffolds: Mechanical Reinforcement and Osteogenic Response.Polymers · 2026Article
- (Ba,Ca)(Ti,Sn)OFrontiers in cellular neuroscience · 2026Article
- Synergistic effects of berberine and adipose stem cells in a silk fibroin-PEG hydrogel for diabetic wound healing via macrophage polarization modulation.Regenerative biomaterials · 2026Article
- Recent Advances in Silk Fibroin Derived fromJournal of functional biomaterials · 2025Review
- Engineering Osteosarcoma In Vitro: From Traditional Models to Biofabricated Platforms for Precision Medicine.ACS omega · 2025Review
- Phillygenin Improves the Focal Adhesion Kinase/Glycogen Synthase Kinase 3β/β-Catenin Axis to Promote Mesenchymal Stem Cell Osteogenic Differentiation.Phytotherapy research : PTR · 2025Article
- Cell-Free Fat Extract with Anti-melanogenic Property Improves the Appearance of Infraorbital Dark Circles: A Preliminary Clinical Study.Aesthetic plastic surgery · 2025Article
- Mechanical and Physical Characterization of a Biphasic 3D Printed Silk-Infilled Scaffold for Osteochondral Tissue Engineering.ACS biomaterials science & engineering · 2024Article
- Impact of Hydroxyapatite on Gelatin/Oxidized Alginate 3D-Printed Cryogel Scaffolds.Gels (Basel, Switzerland) · 2024Article
- Effects of polylactic acid scaffolds with various orientations and diameters on osteogenesis and angiogenesis.Frontiers in bioengineering and biotechnology · 2024Article
- Biomaterials / bioinks and extrusion bioprinting.Bioactive materials · 2023Review
- Aligned Ice Templated Biomaterial Strategies for the Musculoskeletal System.Advanced healthcare materials · 2023Review
- Porous biomaterial scaffolds for skeletal muscle tissue engineering.Frontiers in bioengineering and biotechnology · 2023Review
- Integrating physicomechanical and biological strategies for BTE: biomaterials-induced osteogenic differentiation of MSCs.Theranostics · 2023Review
- Novel Approaches and Biomaterials for Bone Tissue Engineering: A Focus on Silk Fibroin.Materials (Basel, Switzerland) · 2022Review
- Electron Beam-Treated Enzymatically Mineralized Gelatin Hydrogels for Bone Tissue Engineering.Journal of functional biomaterials · 2021Article
- Effect of Polymeric Matrix Stiffness on Osteogenic Differentiation of Mesenchymal Stem/Progenitor Cells: Concise Review.Polymers · 2021Review
Corrections and comments
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Authors and funding
13 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe physical factors of the extracellular matrix have a profound influence on the differentiation behavior of mesenchymal stem cells. In this study, the effect of the biophysical microenvironment on rat bone marrow mesenchymal stem cell (BMSC) osteogenesis was studied both in vitro and in vivo.
methodsTo prepare cell culture scaffolds of varying stiffness, increasing amounts of hydroxyapatite (HAp) were mixed into a polyethylene glycol/silk fibroin (PEG/SF) solution. The amount of HAp ranged from 25 to 100 mg, which provided for different ratios between HAp and the PEG/SF composite. In vitro, the effect of stiffness on the osteogenic differentiation of rat BMSCs was studied. The outcome measures, which were verified in vivo, included the protein expression of runt-related transcription factor 2 and osteocalcin, alkaline phosphatase activity, and the mRNA expression of osteogenesis-related markers.
resultsIncreasing amounts of HAp resulted in an increased elastic modulus of the cell culture scaffolds. The PEG/SF/HAp fabricated with HAp (50 mg) significantly increased cell adhesion and viability (p < 0.05) as well as the expression of all the osteogenesis-related markers (p < 0.05).
conclusionsWe developed a novel cell culture scaffold and demonstrated that substrate stiffness influenced the osteogenic differentiation of rat BMSCs.
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