ArticleJournal of ethnopharmacology2021
In vitro reduction of Plasmodium falciparum gametocytes: Artemisia spp. tea infusions vs. artemisinin.
Article in Journal of ethnopharmacology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 22 citations in OpenAlex.
- Phytochemical Composition of Clonally PropagatedMolecules (Basel, Switzerland) · 2026Article
- Molecular mechanisms involved inFrontiers in microbiology · 2026Review
- Gene Expression, Docking and Machine Learning in Malaria Drug Discovery: A Systematic Review.Biochemistry research international · 2026Review
- Antiplasmodial Activity of Methylangolensate FromJournal of tropical medicine · 2026Article
- Assessment of the in vitro activity and selectivity of Artemisia afra and Artemisia annua aqueous extracts against artemisinin-resistant Plasmodium falciparum.Malaria journal · 2025Article
- Exploring the Phytochemical Diversity and Anti-Plasmodial Potential ofMolecules (Basel, Switzerland) · 2025Article
- The plant matrix of Artemisia annua L. for the treatment of malaria: Pharmacodynamic and pharmacokinetic studies.PloS one · 2025Article
- Medicinal plants as more sustainable therapeutic solutions:Global health economics and sustainability · 2025Article
- Dihydroartemisinin suppresses the susceptibility of Anopheles stephensi to Plasmodium yoelii by activating the Toll signaling pathway.Parasites & vectors · 2024Article
- Artemisinin as a therapeuticNatural product reports · 2023Review
- Knowledge about Asymptomatic Malaria and Acceptability of UsingInternational journal of environmental research and public health · 2023Article
- Secondary Metabolites Isolated fromMetabolites · 2023Review
- Artemisinin-independent inhibitory activity ofLife science alliance · 2022Article
- Review
- Article
- In vitro analyses of Artemisia extracts on Plasmodium falciparum suggest a complex antimalarial effect.PloS one · 2021Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
ethnopharmacological relevanceArtemisia annua has a long history of use in Southeast Asia where it was used to treat "fever", and A. afra has a similar history in southern Africa. Since their discovery, A. annua use, in particular, has expanded globally with millions of people using the plant in therapeutic tea infusions, mainly to treat malaria. AIM OF THE STUDY: In this study, we used in vitro studies to query if and how A. annua and A. afra tea infusions being used across the globe affect asexual Plasmodium falciparum parasites, and their sexual gametocytes. MATERIALS AND
methodsP. falciparumstrain NF54 was grown in vitro, synchronized, and induced to form gametocytes using N-acetylglucosamine. Cultures during asexual, early, and late stage gametocytogenesis were treated with artemisinin, methylene blue, and A. annua and A. afra tea infusions (5 g DW/L) using cultivars that contained 0-283 μM artemisinin. Asexual parasitemia and gametocytemia were analyzed microscopically. Gametocyte morphology also was scored. Markers of early (PfGEXP5) and late stage (Pfs25) gametocyte gene expression also were measured using RT-qPCR.
resultsBoth A. annua and A. afra tea infusions reduced gametocytemia in vitro, and the effect was mainly artemisinin dependent. Expression levels of both marker genes were reduced and also occurred with the effect mainly attributed to artemisinin content of four tested Artemisia cultivars. Tea infusions of both species also inhibited asexual parasitemia and although mainly artemisinin dependent, there was a weak antiparasitic effect from artemisinin-deficient A. afra.
conclusionsThese results showed that A. annua and to a lesser extent, A. afra, inhibited parasitemia and gametocytemia in vitro.
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