ArticleNucleic acids research2020
Plasmid replication-associated single-strand-specific methyltransferases.
Article in Nucleic acids research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- The Single-Stranded DNA Platform: A Potential Broad-Spectrum Vector for Editing Genes in Prokaryotes: ssDNA May Break Through the Difficulties of Genetic Manipulation on Non-Model Bacteria.BioEssays : news and reviews in molecular, cellular and developmental biology · 2026Review
- Plasmid-encoded host reprogramming promotes plasmid dissemination.bioRxiv : the preprint server for biology · 2026Article
- Selective elimination of donor bacteria to analyze plasmid reactions during conjugative transfer.Biology methods & protocols · 2026Review
- Biology of host-dependent restriction-modification in prokaryotes.EcoSal Plus · 2025Review
- Selective elimination of donor bacteria enables global profiling of plasmid gene expression at early stages of conjugation.Nucleic acids research · 2025Article
- The extended mobility of plasmids.Nucleic acids research · 2025Review
- Various plasmid strategies limit the effect of bacterial restriction-modification systems against conjugation.Nucleic acids research · 2024Article
- Article
- Restriction-modification systems have shaped the evolution and distribution of plasmids across bacteria.Nucleic acids research · 2023Article
- Recipient UvrD helicase is involved in single- to double-stranded DNA conversion during conjugative plasmid transfer.Nucleic acids research · 2023Article
- Towards a better understanding of antimicrobial resistance dissemination: what can be learnt from studying model conjugative plasmids?Military Medical Research · 2022Review
- Genome archaeology of two laboratory Salmonella enterica enterica sv Typhimurium.G3 (Bethesda, Md.) · 2021Article
- Context-dependent roles of YAP/TAZ in stem cell fates and cancer.Cellular and molecular life sciences : CMLS · 2021Review
- The Biological Function, Mechanism, and Clinical Significance of m6A RNA Modifications in Head and Neck Carcinoma: A Systematic Review.Frontiers in cell and developmental biology · 2021Review
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Analysis of genomic DNA from pathogenic strains of Burkholderia cenocepacia J2315 and Escherichia coli O104:H4 revealed the presence of two unusual MTase genes. Both are plasmid-borne ORFs, carried by pBCA072 for B. cenocepacia J2315 and pESBL for E. coli O104:H4. Pacific Biosciences SMRT sequencing was used to investigate DNA methyltransferases M.BceJIII and M.EcoGIX, using artificial constructs. Mating properties of engineered pESBL derivatives were also investigated. Both MTases yield promiscuous m6A modification of single strands, in the context SAY (where S = C or G and Y = C or T). Strikingly, this methylation is asymmetric in vivo, detected almost exclusively on one DNA strand, and is incomplete: typically, around 40% of susceptible motifs are modified. Genetic and biochemical studies suggest that enzyme action depends on replication mode: DNA Polymerase I (PolI)-dependent ColE1 and p15A origins support asymmetric modification, while the PolI-independent pSC101 origin does not. An MTase-PolI complex may enable discrimination of PolI-dependent and independent plasmid origins. M.EcoGIX helps to establish pESBL in new hosts by blocking the action of restriction enzymes, in an orientation-dependent fashion. Expression and action appear to occur on the entering single strand in the recipient, early in conjugal transfer, until lagging-strand replication creates the double-stranded form.
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