ArticleThe Science of the total environment2021
Recovering coronavirus from large volumes of water.
Article in The Science of the total environment, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 25 citations in OpenAlex.
- Comparative virome analysis of lake and domestic wastewater revealed the unexpected presence of swine acute diarrhea syndrome coronavirus andFrontiers in microbiology · 2026Article
- Article
- An improved method for determining frequency of multiple variants of SARS-CoV-2 in wastewater using qPCR assays.The Science of the total environment · 2023Article
- COVID-19 and Water Variables: Review and Scientometric Analysis.International journal of environmental research and public health · 2023Review
- Assessment of two volumetrically different concentration approaches to improve sensitivities for SARS-CoV-2 detection during wastewater monitoring.Journal of virological methods · 2023Article
- Evaluation of SARS-CoV-2 concentrations in wastewater and river water samples.Case studies in chemical and environmental engineering · 2022Article
- A Methodological Approach to Water Concentration to Investigate the Presence of SARS-CoV-2 RNA in Surface Freshwaters.Pathogens (Basel, Switzerland) · 2022Article
- Concentration techniques tailored for the detection of SARS-CoV-2 genetic material in domestic wastewater and treatment plant sludge: A review.Journal of environmental chemical engineering · 2021Review
- The impact of coronavirus SARS-CoV-2 (COVID-19) in water: potential risks.Environmental science and pollution research international · 2021Review
- Performance evaluation of a dead-end hollowfiber ultrafiltration method for enumeration of somatic and F+ coliphage from recreational waters.Journal of virological methods · 2021Article
- Factors influencing recovery of SARS-CoV-2 RNA in raw sewage and wastewater sludge using polyethylene glycol-based concentration method.Journal of biomolecular techniques : JBT · 2021Article
- Methods Evaluation for Rapid Concentration and Quantification of SARS-CoV-2 in Raw Wastewater Using Droplet Digital and Quantitative RT-PCR.Food and environmental virology · 2021Article
- Occurrence and Accumulation of Human Enteric Viruses and Phages in Process Water from the Fresh Produce Industry.Foods (Basel, Switzerland) · 2021Article
- Development of a large volume concentration method for recovery of coronavirus from wastewater.The Science of the total environment · 2021Article
- Impacts of COVID-19 pandemic on the wastewater pathway into surface water: A review.The Science of the total environment · 2021Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The need for monitoring tools to better control the ongoing coronavirus disease (COVID-19) pandemic is extremely urgent and the contamination of water resources by excreted viral particles poses alarming questions to be answered. As a first step to overcome technical limitations in monitoring SARS-CoV-2 along the water cycle, we assessed the analytical performance of a dead end hollow fiber ultrafiltration coupled to different options for secondary concentrations to concentrate viral particles from large volume of spiked tap water, seawater and surface water together with two quantitative RT-qPCR detection kits. Spiking the porcine epidemic diarrhea virus (PEDV), an enveloped virus surrogate for SARS-CoV-2, together with the mengovirus, we demonstrated that PEG-precipitation and SENS-kit better recovered PEDV (13.10 ± 0.66%) from tap water, while centrifugal filtration resulted the best option to recover mengovirus regardless of the detection kit used. No statistical significant differences were found when comparing high (10,000 ×g) and low (3500 ×g) centrifugation speeds for the secondary PEG- based concentration of spiked seawater, while considerable inhibition was observed for both viruses detected by NoInh-kit assay. Similarly, the co-concentration of PCR inhibitors and viral particles was observed in surface waters detected with either SENS-kit or NoInh-kit and RNA dilution was needed to achieve acceptable recoveries at the expenses of the overall sensitivity of the method. These methodologies represent suitable options to investigate SARS-CoV-2 occurrence in different water resources and allow to conduct on site sampling of large volume of water.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.