Evidence map›Paper›PMID 33266498›Full record

ArticleAntibodies (Basel, Switzerland)2020

Crystal Structure and Characterization of Human Heavy-Chain Only Antibodies Reveals a Novel, Stable Dimeric Structure Similar to Monoclonal Antibodies.

Carl Mieczkowski, Soheila Bahmanjah, Yao Yu, Jeanne Baker, Gopalan Raghunathan, Daniela Tomazela, Mark Hsieh, Mark McCoy, Corey Strickland, Laurence Fayadat-Dilman

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Antibodies (Basel, Switzerland), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.2field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. The dispensability of VFrontiers in molecular biosciences · 2024
    Article
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Carl MieczkowskiDiscovery Biologics, Protein Sciences, Merck & Co., Inc., South San Francisco, CA 94080, USA.
Soheila BahmanjahDepartment of Chemistry, Modeling and Informatics, Merck & Co., Inc., Kenilworth, NJ 07033, USA.
Yao YuDiscovery Biologics, Protein Sciences, Merck & Co., Inc., South San Francisco, CA 94080, USA.
Jeanne BakerDiscovery Biologics, Protein Sciences, Merck & Co., Inc., South San Francisco, CA 94080, USA.
Gopalan RaghunathanDiscovery Biologics, Protein Sciences, Merck & Co., Inc., South San Francisco, CA 94080, USA.
Daniela TomazelaDiscovery Biologics, Protein Sciences, Merck & Co., Inc., South San Francisco, CA 94080, USA.
Mark HsiehDiscovery Biologics, Protein Sciences, Merck & Co., Inc., South San Francisco, CA 94080, USA.
Mark McCoyDepartment of Pharmacology, Mass Spectrometry & Biophysics, Merck & Co., Inc., Kenilworth, NJ 07033, USA.
Corey StricklandDepartment of Chemistry, Modeling and Informatics, Merck & Co., Inc., Kenilworth, NJ 07033, USA.
Laurence Fayadat-DilmanDiscovery Biologics, Protein Sciences, Merck & Co., Inc., South San Francisco, CA 94080, USA.
Merck & Co., Inc., Rahway, NJ, USA (United States) · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We report the novel crystal structure and characterization of symmetrical, homodimeric humanized heavy-chain-only antibodies or dimers (HC2s). HC2s were found to be significantly coexpressed and secreted along with mAbs from transient CHO HC/LC cotransfection, resulting in an unacceptable mAb developability attribute. Expression of full-length HC2s in the absence of LC followed by purification resulted in HC2s with high purity and thermal stability similar to conventional mAbs. The V

Indexed as

antibodycrystal structureheavy-chain antibodyheavy-chain dimer

Identifiers

PMID33266498
PMCPMC7709113
OpenAlexW3109561424

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.