Evidence map›Paper›PMID 33266157›Full record

ReviewBiology2020

Digital PCR: What Relevance to Plant Studies?

Caterina Morcia, Roberta Ghizzoni, Chiara Delogu, Lorella Andreani, Paola Carnevali, Valeria Terzi

Abstract readReview
In one paragraph

Review in Biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed.

  1. Article
  2. Digital PCR for the Authentication of KAMUTFoods (Basel, Switzerland) · 2026
    Article
  3. Article
  4. Early Detection of BothInternational journal of molecular sciences · 2024
    Article
  5. Towards Pathogen-Free Coconut Germplasm Exchange.Plants (Basel, Switzerland) · 2024
    Review
  6. Article
  7. Article
  8. Article
  9. Review
  10. Article
  11. Article
  12. Article
  13. Phytoplasma diseases of plants: molecular diagnostics and way forward.World journal of microbiology & biotechnology · 2021
    Review
  14. Article
  15. Article
  16. Review
  17. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Caterina MorciaConsiglio per la Ricerca in Agricoltura e L'analisi Dell'economia Agraria-Centro di Ricerca Genomica e Bioinformatica (CREA-GB), via San Protaso 302, 29017 Fiorenzuola d'Arda, Italy.ORCID 0000-0001-5316-7435
Roberta GhizzoniConsiglio per la Ricerca in Agricoltura e L'analisi Dell'economia Agraria-Centro di Ricerca Genomica e Bioinformatica (CREA-GB), via San Protaso 302, 29017 Fiorenzuola d'Arda, Italy.
Chiara DeloguConsiglio per la Ricerca in Agricoltura e L'analisi Dell'economia Agraria-Centro di Ricerca Difesa e Certificazione (CREA-DC), via Emilia km 307, 26838 Tavazzano, Italy.
Lorella AndreaniConsiglio per la Ricerca in Agricoltura e L'analisi Dell'economia Agraria-Centro di Ricerca Difesa e Certificazione (CREA-DC), via Emilia km 307, 26838 Tavazzano, Italy.
Paola CarnevaliBarilla S.p.A., via Mantova 166, I-43122 Parma, Italy.
Valeria TerziConsiglio per la Ricerca in Agricoltura e L'analisi Dell'economia Agraria-Centro di Ricerca Genomica e Bioinformatica (CREA-GB), via San Protaso 302, 29017 Fiorenzuola d'Arda, Italy.ORCID 0000-0002-8006-6805

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Digital PCR (dPCR) is a breakthrough technology that able to provide sensitive and absolute nucleic acid quantification. It is a third-generation technology in the field of nucleic acid amplification. A unique feature of the technique is that of dividing the sample into numerous separate compartments, in each of which an independent amplification reaction takes place. Several instrumental platforms have been developed for this purpose, and different statistical approaches are available for reading the digital output data. The dPCR assays developed so far in the plant science sector were identified in the literature, and the major applications, advantages, disadvantages, and applicative perspectives of the technique are presented and discussed in this review.

Indexed as

copy number variationdiagnosticsdigital PCRgene expressiongenetically modified organismsgenetic traceabilityspecies

Identifiers

PMID33266157
PMCPMC7760125

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.