ArticleFoods (Basel, Switzerland)2020
A Novel Method for Antibiotic Detection in Milk Based on Competitive Magnetic Immunodetection.
Article in Foods (Basel, Switzerland), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 37 citations in OpenAlex.
- Ultrasensitive and Real-Time Detection of Kanamycin Residues in Milk Using an Aptasensor Based on Microfluidic Capacitive Strategy.Biosensors · 2025Article
- Fundamentals and Applications of Dual-Frequency Magnetic Particle Spectroscopy: Review for Biomedicine and Materials Characterization.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Miniaturized Pathogen Detection System Using Magnetic Nanoparticles and Microfluidics Technology.Micromachines · 2024Article
- Key Contributors to Signal Generation in Frequency Mixing Magnetic Detection (FMMD): An In Silico Study.Sensors (Basel, Switzerland) · 2024Article
- Pen direct writing of SERRS-based lateral flow assays for detection of penicillin G in milk.Nanoscale advances · 2024Article
- Magnetic Particle Spectroscopy for Point-of-Care: A Review on Recent Advances.Sensors (Basel, Switzerland) · 2023Review
- Frequency Mixing Magnetic Detection Setup Employing Permanent Ring Magnets as a Static Offset Field Source.Sensors (Basel, Switzerland) · 2022Article
- Implications of Veterinary Medicine in the comprehension and stewardship of antimicrobial resistance phenomenon. From the origin till nowadays.Veterinary and animal science · 2022Article
- Magnetic nanoparticles and magnetic particle spectroscopy-based bioassays: a 15 year recap.Nano futures · 2022Article
- Novel Analytical Methods in Food Analysis.Foods (Basel, Switzerland) · 2022Article
- Establishment and Validation of a GC-MS/MS Method for the Quantification of Penicillin G Residues in Poultry Eggs.Foods (Basel, Switzerland) · 2021Article
- Multiplex Detection of Magnetic Beads Using Offset Field Dependent Frequency Mixing Magnetic Detection.Sensors (Basel, Switzerland) · 2021Article
- Comparative Modeling of Frequency Mixing Measurements of Magnetic Nanoparticles Using Micromagnetic Simulations and Langevin Theory.Nanomaterials (Basel, Switzerland) · 2021Article
- Development of Fast and Portable Frequency Magnetic Mixing-Based Serological SARS-CoV-2-Specific Antibody Detection Assay.Frontiers in microbiology · 2021Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The misuse of antibiotics as well as incorrect dosage or insufficient time for detoxification can result in the presence of pharmacologically active molecules in fresh milk. Hence, in many countries, commercially available milk has to be tested with immunological, chromatographic or microbiological analytical methods to avoid consumption of antibiotic residues. Here a novel, sensitive and portable assay setup for the detection and quantification of penicillin and kanamycin in whole fat milk (WFM) based on competitive magnetic immunodetection (cMID) is described and assay accuracy determined. For this, penicillin G and kanamycin-conjugates were generated and coated onto a matrix of immunofiltration columns (IFC). Biotinylated penicillin G or kanamycin-specific antibodies were pre-incubated with antibiotics-containing samples and subsequently applied onto IFC to determine the concentration of antibiotics through the competition of antibody-binding to the antibiotic-conjugate molecules. Bound antibodies were labeled with streptavidin-coated magnetic particles and quantified using frequency magnetic mixing technology. Based on calibration measurements in WFM with detection limits of 1.33 ng·mL
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.