ArticleCell reports2020
Optogenetic Tuning Reveals Rho Amplification-Dependent Dynamics of a Cell Contraction Signal Network.
Article in Cell reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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Who cites it
26 citing papers in PubMed, 63 citations in OpenAlex.
- The ROCK1 PHC1 domain mediates recruitment to Rho activity zones and contractility signaling.Life science alliance · 2026Article
- Delineation of signaling microdomains from live-cell movies.Cell systems · 2026Article
- Non-canonical and constitutive activation of small GTPases: more than an exception to the rule?The Biochemical journal · 2026Review
- Modeling mechanochemical coupling in optogenetically activated cell layers.Biophysical journal · 2026Article
- Using Floquet theory to unravel far-from equilibrium dynamics in reaction-diffusion systems.PNAS nexus · 2026Article
- Research progress on ferroptosis in drug resistance and therapy of gastric cancer.Journal of cancer research and clinical oncology · 2025Review
- Optogenetic stimulation of Lbc GEF-mediated Rho activity dynamics promotes cell invasion.Molecular biology of the cell · 2025Article
- Guanine Nucleotide Exchange Factors and Small GTPases: Their Regulation and Functions, Diseases, and Therapeutic Targets.MedComm · 2025Review
- Cross-talk between Rac and Rap GTPases in migrating cells.Molecular biology of the cell · 2025Article
- Optogenetic control of a GEF of RhoA uncovers a signaling switch from retraction to protrusion.eLife · 2025Article
- Analysis of the spatio-temporal dynamics of a Rho-GEF-H1-myosin activator-inhibitor reaction-diffusion system.Royal Society open science · 2025Article
- Excitable Rho dynamics control cell shape and motility by sequentially activating ERM proteins and actomyosin contractility.Science advances · 2024Article
- Mechanical and biochemical feedback combine to generate complex contractile oscillations in cytokinesis.Current biology : CB · 2024Article
- Patterning of the cell cortex by Rho GTPases.Nature reviews. Molecular cell biology · 2024Review
- Rho GTPase activity crosstalk mediated by Arhgef11 and Arhgef12 coordinates cell protrusion-retraction cycles.Nature communications · 2023Article
- Direct investigation of cell contraction signal networks by light-based perturbation methods.Pflugers Archiv : European journal of physiology · 2023Review
- Shining a light on RhoA: Optical control of cell contractility.The international journal of biochemistry & cell biology · 2023Review
- Mechanosensitive stem cell fate choice is instructed by dynamic fluctuations in activation of Rho GTPases.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Cell size and actin architecture determine force generation in optogenetically activated cells.Biophysical journal · 2023Article
- Light-driven biological actuators to probe the rheology of 3D microtissues.Nature communications · 2023Article
Corrections and comments
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Authors and funding
13 authors at 8 institutions in 7 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Local cell contraction pulses play important roles in tissue and cell morphogenesis. Here, we improve a chemo-optogenetic approach and apply it to investigate the signal network that generates these pulses. We use these measurements to derive and parameterize a system of ordinary differential equations describing temporal signal network dynamics. Bifurcation analysis and numerical simulations predict a strong dependence of oscillatory system dynamics on the concentration of GEF-H1, an Lbc-type RhoGEF, which mediates the positive feedback amplification of Rho activity. This prediction is confirmed experimentally via optogenetic tuning of the effective GEF-H1 concentration in individual living cells. Numerical simulations show that pulse amplitude is most sensitive to external inputs into the myosin component at low GEF-H1 concentrations and that the spatial pulse width is dependent on GEF-H1 diffusion. Our study offers a theoretical framework to explain the emergence of local cell contraction pulses and their modulation by biochemical and mechanical signals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.