Evidence map›Paper›PMID 33260683›Full record

ArticleInternational journal of molecular sciences2020

AKAP12 Supports Blood-Brain Barrier Integrity against Ischemic Stroke.

Ji Hae Seo, Takakuni Maki, Nobukazu Miyamoto, Yoon Kyong Choi, Kelly K Chung, Gen Hamanaka, Ji Hyun Park, Emiri T Mandeville, Hajime Takase, Kazuhide Hayakawa and 5 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed, 1 pooled it
1.0field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 1 synthesis or guideline pooled it, 26 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Article
  4. Review
  5. Article
  6. Article
  7. Article
  8. Article
  9. Plasma cell-free RNA signatures of inflammatory syndromes in children.Proceedings of the National Academy of Sciences of the United States of America · 2024
    Article
  10. Article
  11. Article
  12. Article
  13. Role of A-Kinase Anchoring Protein 12 in the Central Nervous System.Journal of clinical neurology (Seoul, Korea) · 2023
    Review
  14. Review
  15. Wnt Signaling in Inner Blood-Retinal Barrier Maintenance.International journal of molecular sciences · 2021
    Review
  16. Review
  17. Review
  18. GoatFrontiers in genetics · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 3 institutions in 2 countries.

Ji Hae SeoNeuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital and Harvard Medical School, Charlestown, Boston, MA 02129, USA.
Takakuni MakiNeuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital and Harvard Medical School, Charlestown, Boston, MA 02129, USA.
Nobukazu MiyamotoNeuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital and Harvard Medical School, Charlestown, Boston, MA 02129, USA.ORCID 0000-0003-3037-169X
Yoon Kyong ChoiNeuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital and Harvard Medical School, Charlestown, Boston, MA 02129, USA.ORCID 0000-0003-0242-9612
Kelly K ChungNeuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital and Harvard Medical School, Charlestown, Boston, MA 02129, USA.
Gen HamanakaNeuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital and Harvard Medical School, Charlestown, Boston, MA 02129, USA.
Ji Hyun ParkNeuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital and Harvard Medical School, Charlestown, Boston, MA 02129, USA.
Emiri T MandevilleNeuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital and Harvard Medical School, Charlestown, Boston, MA 02129, USA.
Hajime TakaseNeuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital and Harvard Medical School, Charlestown, Boston, MA 02129, USA.
Kazuhide HayakawaNeuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital and Harvard Medical School, Charlestown, Boston, MA 02129, USA.
Josephine LokNeuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital and Harvard Medical School, Charlestown, Boston, MA 02129, USA.
Irwin H GelmanDepartment of Cancer Genetics and Genomics, Roswell Park Comprehensive Cancer Center, Buffalo, NY 14203, USA.ORCID 0000-0001-9204-207X
Kyu-Won KimCollege of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 08826, Korea.
Eng H LoNeuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital and Harvard Medical School, Charlestown, Boston, MA 02129, USA.
Ken AraiNeuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital and Harvard Medical School, Charlestown, Boston, MA 02129, USA.
Harvard University · USRoswell Park Comprehensive Cancer Center · USSeoul National University · KR

Funding

Oligo-Vascular Signaling in StrokeR01NS065089 · NINDS · MASSACHUSETTS GENERAL HOSPITAL · PI ARAI, KEN · 2010 to 2019
$3.8M
Oligodendrocyte Precursor Cells Regulate White Matter Remodeling in Vascular Cognitive Impairment and DementiaR01NS113556 · NINDS · MASSACHUSETTS GENERAL HOSPITAL · PI ARAI, KEN · 2019 to 2023
$2.4M
Neuregulin-1 decreases endothelial hyper-permeability and microthrombosis after traumatic brain injuryR01NS091573 · NINDS · MASSACHUSETTS GENERAL HOSPITAL · PI LOK, JOSEPHINE MUN-YEE · 2015 to 2019
$1.9M
Aging dampens compensatory angiogenesis via downregulation of VEGF signaling in subcortical ischemic vascular dementiaR21AG066478 · NIA · MASSACHUSETTS GENERAL HOSPITAL · PI ARAI, KEN · 2020 to 2020
$453k
NIA NIH HHS R21 AG066478NIH HHS R01NS065089; R01NS113556NINDS NIH HHS R01 NS065089NINDS NIH HHS R01 NS091573NINDS NIH HHS R01 NS113556
6 · The paper itself

Abstract

A-kinase anchor protein 12 (AKAP12) is a scaffolding protein that associates with intracellular molecules to regulate multiple signal transductions. Although the roles of AKAP12 in the central nervous system are still relatively understudied, it was previously shown that AKAP12 regulates blood-retinal barrier formation. In this study, we asked whether AKAP12 also supports the function and integrity of the blood-brain barrier (BBB). In a mouse model of focal ischemia, the expression level of AKAP12 in cerebral endothelial cells was upregulated during the acute phase of stroke. Also, in cultured cerebral endothelial cells, oxygen-glucose deprivation induced the upregulation of AKAP12. When AKAP12 expression was suppressed by an siRNA approach in cultured endothelial cells, endothelial permeability was increased along with the dysregulation of ZO-1/Claudin 5 expression. In addition, the loss of AKAP12 expression caused an upregulation/activation of the Rho kinase pathway, and treatment of Rho kinase inhibitor Y-27632 mitigated the increase of endothelial permeability in AKAP12-deficient endothelial cell cultures. These in vitro findings were confirmed by our in vivo experiments using

Indexed as

A Kinase Anchor ProteinsAnimalsBlood-Brain BarrierCell Cycle ProteinsCell Membrane PermeabilityEndothelium, VascularIschemic StrokeMice, Inbred C57BLMice, Knockoutrho-Associated KinasesAkap12 protein, mouseA Kinase Anchor ProteinsCell Cycle Proteinsrho-Associated KinasesA-kinase anchor protein 12blood-brain barrierendothelial cellRho kinasestroke

Identifiers

PMID33260683
PMCPMC7730430
OpenAlexW3109401851

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.