ArticleJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology2021
Single-step RT-PCR assay for dual genotyping of GI and GII norovirus strains.
Article in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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38 citing papers in PubMed, 59 citations in OpenAlex.
- Evaluation of false positive enteric viruses results from the BIOFIRE® FILMARRAY® gastrointestinal PCR panel.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2026Article
- Emergence of norovirus GII.7 and predominance of recombinant strains in pediatric acute gastroenteritis in Japan, 2021-2024.Archives of virology · 2026Article
- Human norovirus GII genotypic diversity in Gwangju, South Korea, based on ORF1-ORF2 junction (RdRp-VP1) analysis from 2020 to 2024.Scientific reports · 2026Article
- Unraveling the Transmission Dynamics of a Novel Norovirus GII.17[P17] Lineage During Two Consecutive Outbreaks in a Spanish Hospital.Journal of medical virology · 2026Article
- Emergence and predominance of GII.17[P17] noroviruses in Brazil, 2023-2024.Scientific reports · 2026Article
- Clinical, Molecular and Evolutionary Characterization of Noroviruses in Hospitalized Children With Acute Gastroenteritis in Vietnam, 2016 to 2021.Journal of medical virology · 2026Article
- A sixteen-month monitoring of human enteric viruses associated with acute gastroenteritis in Senegal.Gut pathogens · 2026Article
- Article
- Metagenomic analysis and genomic characterization of enterovirus A76 and Norovirus GI.6[P11] co-infection in a patient with acute gastroenteritis in Thailand.Scientific reports · 2025Article
- Molecular Epidemiology of Norovirus Outbreaks in Childcare Centers and Schools in South Korea in 2023.Journal of microbiology and biotechnology · 2025Article
- Norovirus GI.5 [P4]: first report of the rare norovirus recombinant variant in Northeastern Mexico and its global epidemiological context.Virus genes · 2025Article
- Article
- A Decade of Chronic Norovirus Infection Surveillance at the National Institutes of Health Clinical Research Center: Clinical Characteristics, Molecular Epidemiology, and Replication.The Journal of infectious diseases · 2025Article
- Foodborne acute gastroenteritis outbreak associated with a rare norovirus recombinant GII.10[P16] genotype, Brazil, 2023.Memorias do Instituto Oswaldo Cruz · 2025Article
- Emergence of norovirus GII.17[P16] in adult patients with acute gastroenteritis in Thailand during 2021-2023.PloS one · 2025Article
- Molecular Characterization of Noroviruses Causing Acute Gastroenteritis Outbreaks among US Military Recruits, 2013-2023.Emerging infectious diseases · 2024Article
- Prevalence and genotype distribution of norovirus in Ningxia Hui Autonomous Region, China, from 2011 to 2022.Virology journal · 2024Article
- Epidemiological Features of Human Norovirus Genotypes before and after COVID-19 Countermeasures in Osaka, Japan.Viruses · 2024Article
- An unusual diarrheal outbreak in the community in Eastern Thailand caused by Norovirus GII.3[P25].Virology journal · 2024Article
- Etiology and Epidemiology of Travelers' Diarrhea among US Military and Adult Travelers, 2018-2023.Emerging infectious diseases · 2024Article
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Authors and funding
10 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundNoroviruses are the major cause of acute gastroenteritis (AGE) in people of all ages globally. Standardized genotyping is key for outbreak investigations and surveillance networks.
objectiveHere we describe the validation of a one-step conventional RT-PCR assay for sequence-based dual typing of GI and GII noroviruses. This polymerase (P) and capsid (C) dual typing assay uses a combination of previously published oligonucleotide primers amplifying a genomic region spanning the 3'-end of ORF1 and 5'end of ORF2 resulting in a 579 bp product for GI and 570 bp product for GII viruses.
resultsThe limit of detection of the assay ranged from 5 to 50 copies of viral RNA per reaction for GI and GII. To validate the assay, we tested 2,663 noroviruspositive stool samples from outbreaks and sporadic cases of AGE in Bangladesh, Guatemala, Peru, and USA collected between 2010-2019, of which 2,392 (90 %) were genotyped successfully. Most of the known genotypes infecting humans (GI (n = 9) and GII (n = 23)) and P types (GI (n = 15), GII, (n = 20)) could be detected. The remaining 270 samples had low viral load (Ct > 30) by real-time RT-PCR. A panel of 166 samples positive for other enteric viruses (rotavirus, astrovirus, sapovirus, adenovirus type 40/41) tested negative.
conclusionThe use of broadly reactive genotyping assays greatly strengthens exchange of standardized genotype data globally to monitor trends in genotype diversity which is important for both the development of vaccines and to measure their impact.
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