ArticleThe ocular surface2021
Creation and grading of experimental corneal scars in mice models.
Article in The ocular surface, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 16 citations in OpenAlex.
- Macrophages increase in corneal epithelium and stroma after photorefractive keratectomy-like corneal abrasion and amplify ocular pain responses in rats.Experimental eye research · 2026Article
- MCOA: A Comprehensive Multimodal Dataset for Advancing Deep Learning in Corneal Opacity Assessment.Scientific data · 2025Article
- Integrated transcriptome and proteome analysis identifies keratins as key regulators of corneal scarring in a murine model.Computational and structural biotechnology journal · 2025Article
- Dysfunctional latent transforming growth factor β activation after corneal injury in a classical Ehlers-Danlos model.Matrix biology : journal of the International Society for Matrix Biology · 2024Article
- Inflammation-suppressing cornea-in-a-syringe with anti-viral GF19 peptide promotes regeneration in HSV-1 infected rabbit corneas.NPJ Regenerative medicine · 2024Article
- Classifications of anterior segment structure of congenital corneal opacity in infants and toddlers by ultrasound biomicroscopy and slit-lamp microscopic photographs: an observational study.BMC ophthalmology · 2024Observational
- Stromal matrix directs corneal fibroblasts to re-express keratocan after injury and transplantation.Disease models & mechanisms · 2023Article
- Effect of Lutein on Ocular Goblet Cell, IFN-γ, and IL-17 Concentration in Dry Eye-Induced Mice Model.Cureus · 2023Article
- Collagen XII regulates stromal wound closure.Experimental eye research · 2023Article
- Genipin increases extracellular matrix synthesis preventing corneal perforation.The ocular surface · 2023Article
- Transcriptomic Profiling of Human Limbus-Derived Stromal/Mesenchymal Stem Cells-Novel Mechanistic Insights into the Pathways Involved in Corneal Wound Healing.International journal of molecular sciences · 2022Article
- Local bone metabolism balance regulation via double-adhesive hydrogel for fixing orthopedic implants.Bioactive materials · 2022Article
- Downregulation of collagen XI during late postnatal corneal development is followed by upregulation after injury.Journal of cell science · 2022Article
- Collagen XIV Is an Intrinsic Regulator of Corneal Stromal Structure and Function.The American journal of pathology · 2021Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
purposeTo develop a stromal wound healing model and a reliable scar classification score system that correlates photographic evaluation with changes in the structure and organization of the extracellular matrix. MATERIALS AND
methodsWe tested three stromal injury techniques in adult C57BL/6 mice. Technique 1, a lineal partial thickness keratotomy in the horizontal axis. Technique 2, corneal epithelial and stromal debridement using a diamond burr in the horizontal axis, and technique 3, a combination of techniques 1 and 2. To assess intra-observer and inter-observer agreement between two examiners evaluating formed stromal scars, stereo microscopic photographs of anterior segment were scored by two masked examiners at around 1-month. Depending on the severity of opacification and the area of involvement, scars were classified on a scale from 0 to 3 based on a modified Fantes haze scale. Extracellular matrix composition as well as matrix organization, macrophage infiltration and neovascularization were evaluated with immunofluorescence and second harmonic generation (SHG) microscopy.
resultsTechnique 1 created mild scars, with a score of 0.5 ± 0.43, while techniques 2 (score 2.1 ± 0.45) and 3 (score 2 ± 0.66), created dense scars with a higher score. A significant difference in scar severity score was noted between the 3 techniques (one way ANOVA, p < 0.0001). Masked graders demonstrated excellent agreement (intraclass correlation = 0.927 [95% confidence interval: 0.87-0.96]). The severity of scars noted at stereo microscopy correlated with the severity of changes in extracellular matrix in the stroma as demonstrated by the expression of collagens I, IV and fibronectin and evaluation of matrix hierarchical organization. In contrast to mild scarring, moderate and severe scars had increased expression of CD31 and CD68, markers of vascular endothelial cells and macrophages, respectively.
conclusionMouse models of stromal scarring using simple surgical techniques are described. Corneal scars can be consistently classified by two observers. Grading of scar severity positively correlates with changes in extracellular matrix composition, disorganization and cell infiltration.
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