Evidence map›Paper›PMID 33259847›Full record

ArticleJournal of neuroscience methods2021

Fluorescence microendoscopy for in vivo deep-brain imaging of neuronal circuits.

Brenton T Laing, Justin N Siemian, Sarah Sarsfield, Yeka Aponte

Open access · greenAbstract read
In one paragraph

Article in Journal of neuroscience methods, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
2.5field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 17 citations in OpenAlex.

  1. The State of High-Resolution Imaging of the Human Inner Ear: A Look Into the Black Box.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025
    Review
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  3. Review
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  5. Article
  6. Using deep learning to study emotional behavior in rodent models.Frontiers in behavioral neuroscience · 2022
    Review
  7. Anatomical Methods to Study the Suprachiasmatic Nucleus.Methods in molecular biology (Clifton, N.J.) · 2022
    Article
  8. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Brenton T LaingNeuronal Circuits and Behavior Unit, National Institute on Drug Abuse Intramural Research Program, National Institutes of Health, Baltimore, MD, 21224-6823, USA.
Justin N SiemianNeuronal Circuits and Behavior Unit, National Institute on Drug Abuse Intramural Research Program, National Institutes of Health, Baltimore, MD, 21224-6823, USA.
Sarah SarsfieldNeuronal Circuits and Behavior Unit, National Institute on Drug Abuse Intramural Research Program, National Institutes of Health, Baltimore, MD, 21224-6823, USA.
Yeka AponteNeuronal Circuits and Behavior Unit, National Institute on Drug Abuse Intramural Research Program, National Institutes of Health, Baltimore, MD, 21224-6823, USA; The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA. Electronic address: yeka.aponte@nih.gov.
National Institute on Drug Abuse · USNational Institutes of Health · US

Funding

Hypothalamic neuronal circuits controlling survival behaviorsZIADA000595 · NIDA · NATIONAL INSTITUTE ON DRUG ABUSE · PI APONTE, YEKA · 2013 to 2025
$21.3M
Intramural NIH HHS ZIA DA000595
6 · The paper itself

Abstract

Imaging neuronal activity in awake, behaving animals has become a groundbreaking method in neuroscience that has rapidly enhanced our understanding of how the brain works. In vivo microendoscopic imaging has enabled researchers to see inside the brains of experimental animals and thus has emerged as a technology fit to answer many experimental questions. By combining microendoscopy with cutting edge targeting strategies and sophisticated analysis tools, neuronal activity patterns that underlie changes in behavior and physiology can be identified. However, new users may find it challenging to understand the techniques and to leverage this technology to best suit their needs. Here we present a background and overview of the necessary components for performing in vivo optical calcium imaging and offer some detailed guidance for current recommended approaches.

Indexed as

BrainNeuronsAnimalsCalciumMicroscopy, FluorescenceNeuroimagingCalciumFunctional imagingGCaMPGECIGRIN lensMicroendoscopyNeuronal circuits

Identifiers

PMID33259847
PMCPMC8745022
OpenAlexW3109565770

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.