ArticleLasers in surgery and medicine2021
Scattering-Based Light-Sheet Microscopy for Rapid Cellular Imaging of Fresh Tissue.
Article in Lasers in surgery and medicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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9 citing papers in PubMed.
- Axially Swept Light-Sheet Microscopy using scattering and fluorescence contrast mechanisms.Proceedings of SPIE--the International Society for Optical Engineering · 2026Article
- Multi-parametric Photoacoustic Imaging Combined with Acoustic Radiation Force Impulse Imaging for Applications in Tissue Engineering.Annals of biomedical engineering · 2025Article
- Automated analysis of scattering-based light sheet microscopy images of anal squamous intraepithelial lesions.Biomedical optics express · 2024Article
- Compact scattering-based light sheet microscopy probe using a custom miniature objective lens.Journal of optical microsystems · 2024Article
- Scattering-Based Light-Sheet Microscopy Imaging of Human Papillomavirus-Associated Squamous Lesions of the Anal Canal: A Proof-of-Principle Study.Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc · 2024Article
- Volumetric imaging of human mesenchymal stem cells (hMSCs) for non-destructive quantification of 3D cell culture growth.PloS one · 2023Article
- Investigation of different wavelengths for scattering-based light sheet microscopy.Biomedical optics express · 2022Article
- Light-Sheet Scattering Microscopy to Visualize Long-Term Interactions Between Cells and Extracellular Matrix.Frontiers in immunology · 2022Article
- Scattering oblique plane microscopy forBiomedical optics express · 2021Article
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Abstract
BACKGROUND AND
objectivesLight-sheet microscopy (LSM) is a novel imaging technology that has been used for imaging fluorescence contrast in basic life science research. In this paper, we have developed a scattering-based LSM (sLSM) for rapidly imaging the cellular morphology of fresh tissues without any exogenous fluorescent dyes. STUDY DESIGN/MATERIALS AND
methodsIn the sLSM device, a thin light sheet with the central wavelength of 834 nm was incident on the tissue obliquely, 45° relative to the tissue surface. The detection optics was configured to map the light sheet-illuminated area onto a two-dimensional imaging sensor. The illumination numerical aperture (NA) was set as 0.0625, and the detection NA 0.3.
resultsThe sLSM device achieved a light sheet thickness of less than 6.7 µm over 284 µm along the illumination optical axis. The detection optics of the sLSM device had a resolution of 1.8 µm. The sLSM images of the swine kidney ex vivo visualized tubules with similar sizes and shapes to those observed in histopathologic images. The swine duodenum sLSM images revealed cell nuclei and villi architecture in superficial lesions and glands in deeper regions.
conclusionsThe preliminary results suggest that sLSM may have the potential for rapidly examining the freshly-excised tissue ex vivo or intact tissue in vivo at microscopic resolution. Further optimization and performance evaluation of the sLSM technology will be needed in the future. Lasers Surg. Med. © 2020 Wiley Periodicals LLC.
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