Evidence map›Paper›PMID 33259594›Full record

ArticleThe Journal of antimicrobial chemotherapy2021

Repeated isolation of an antibiotic-dependent and temperature-sensitive mutant of Pseudomonas aeruginosa from a cystic fibrosis patient.

Daniel J Wolter, Alison Scott, Catherine R Armbruster, Dale Whittington, John S Edgar, Xuan Qin, Anne Marie Buccat, Sharon McNamara, Marcella Blackledge, Adam Waalkes and 3 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of antimicrobial chemotherapy, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.4field-weighted citation impact, top 50% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 1 country.

Daniel J WolterDepartment of Pediatrics, University of Washington, Seattle, WA, USA.
Alison ScottDepartment of Microbial Pathogenesis, University of Maryland, Baltimore, MD, USA.
Catherine R ArmbrusterDepartment of Microbiology, University of Washington, Seattle, WA, USA.
Dale WhittingtonDepartment of Medicinal Chemistry, University of Washington, Seattle, WA, USA.
John S EdgarDepartment of Medicinal Chemistry, University of Washington, Seattle, WA, USA.
Xuan QinSeattle Children's Hospital, Seattle, WA, USA.
Anne Marie BuccatSeattle Children's Hospital, Seattle, WA, USA.
Sharon McNamaraSeattle Children's Hospital, Seattle, WA, USA.
Marcella BlackledgeSeattle Children's Hospital, Seattle, WA, USA.
Adam WaalkesDepartment of Laboratory Medicine, University of Washington, Seattle, WA, USA.
Stephen J SalipanteDepartment of Laboratory Medicine, University of Washington, Seattle, WA, USA.
Robert K ErnstDepartment of Microbial Pathogenesis, University of Maryland, Baltimore, MD, USA.
Lucas R HoffmanDepartment of Pediatrics, University of Washington, Seattle, WA, USA.
Seattle Children's Hospital · USUniversity of Washington · USUniversity of Maryland, Baltimore · US

Funding

Translational Research Center to Expedite Novel Therapies in Cystic FibrosisP30DK089507 · NIDDK · SEATTLE CHILDREN'S HOSPITAL · PI Christopher Hooper Goss · 2010 to 2026
$21.4M
NIDDK NIH HHS P30 DK089507
6 · The paper itself

Abstract

backgroundBacteria adapt to survive and grow in different environments. Genetic mutations that promote bacterial survival under harsh conditions can also restrict growth. The causes and consequences of these adaptations have important implications for diagnosis, pathogenesis, and therapy.

objectivesWe describe the isolation and characterization of an antibiotic-dependent, temperature-sensitive Pseudomonas aeruginosa mutant chronically infecting the respiratory tract of a cystic fibrosis (CF) patient, underscoring the clinical challenges bacterial adaptations can present.

methodsRespiratory samples collected from a CF patient during routine care were cultured for standard pathogens. P. aeruginosa isolates recovered from samples were analysed for in vitro growth characteristics, antibiotic susceptibility, clonality, and membrane phospholipid and lipid A composition. Genetic mutations were identified by whole genome sequencing.

resultsP. aeruginosa isolates collected over 5 years from respiratory samples of a CF patient frequently harboured a mutation in phosphatidylserine decarboxylase (psd), encoding an enzyme responsible for phospholipid synthesis. This mutant could only grow at 37°C when in the presence of supplemented magnesium, glycerol, or, surprisingly, the antibiotic sulfamethoxazole, which the source patient had repeatedly received. Of concern, this mutant was not detectable on standard selective medium at 37°C. This growth defect correlated with alterations in membrane phospholipid and lipid A content.

conclusionsA P. aeruginosa mutant chronically infecting a CF patient exhibited dependence on sulphonamides and would likely evade detection using standard clinical laboratory methods. The diagnostic and therapeutic challenges presented by this mutant highlight the complex interplay between bacterial adaptation, antibiotics, and laboratory practices, during chronic bacterial infections.

Indexed as

Cystic FibrosisPseudomonas InfectionsAnti-Bacterial AgentsHumansPseudomonas aeruginosaTemperatureAnti-Bacterial Agents

Identifiers

PMID33259594
PMCPMC7879151
OpenAlexW3109299058

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.