ArticlePloS one2020
Biogenic iron oxide nanoparticles enhance callogenesis and regeneration pattern of recalcitrant Cicer arietinum L.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 59 citations in OpenAlex.
- Green synthesis of zanthoxylum rhetsa (roxb.) DC. mediated iron oxide nanoparticles with physicochemical characterisations and in vitro biomedical applications.Discover nano · 2026Article
- Application of Nanoparticles in Plant In Vitro Culture for Micropropagation and Secondary Metabolite Production: A Review.Plants (Basel, Switzerland) · 2026Review
- Multifaceted gold nanoparticles by bark extract of Sweetinia mahagoni and their potential antimicrobial, antioxidant, anticancer and antiviral applications.Scientific reports · 2026Article
- Potential impacts of nanoparticles integration on micropropagation efficiency: current achievements and prospects.Frontiers in plant science · 2025Review
- Effective substitution of ferrous sulfate with iron oxide nanoparticles enhances growth, antioxidant activity, and stevioside accumulation in micro-propagatedFrontiers in plant science · 2025Article
- Expression of mastitis causing fibrinogen binding protein of gram positive bacteria in genetically engineered switchgrass and antibodies production in mice.Physiology and molecular biology of plants : an international journal of functional plant biology · 2024Article
- Green synthesized iron oxide nanoparticles as a potential regulator of callus growth, plant physiology, antioxidative and microbial contamination in Oryza sativa L.BMC plant biology · 2024Article
- Nano-Integrated Plant Tissue Culture to Increase the Rate of Callus Induction, Growth, and Curcuminoid Production inPlants (Basel, Switzerland) · 2024Article
- Boosting Solanum tuberosum resistance to Alternaria solani through green synthesized ferric oxide (FeScientific reports · 2024Article
- Article
- Multifaceted Role of Nanomaterials in Modulating In Vitro Seed Germination, Plant Morphogenesis, Metabolism and Genetic Engineering.Plants (Basel, Switzerland) · 2023Review
- Bifunctional role of some biogenic nanoparticles in controlling wilt disease and promoting growth of common bean.AMB Express · 2023Article
- Biogenic Synthesis of Copper-Based Nanomaterials Using Plant Extracts and Their Applications: Current and Future Directions.Nanomaterials (Basel, Switzerland) · 2022Review
- Effects of biogenic silver and iron nanoparticles on soybean seedlings (Glycine max).BMC plant biology · 2022Article
- Biosynthesis and characterization of iron oxide nanoparticles fromFrontiers in plant science · 2022Article
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Authors and funding
10 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study is the first report on the biosynthesized iron oxide nanoparticles (IONPs) which mediate in-vitro callus induction and shoot regeneration in economically important recalcitrant chickpea crop (Cicer arietinum L.). Here, we used leaf extract of Cymbopogon jwarancusa for the synthesis of IONPs in order to achieve a better biocompatibility. The bioactive compounds in C. jwarancusa leaf extract served as both reducing and capping agents in the fabrication process of IONPs. Field emission scanning electron microscopy (FE-SEM) revealed rods like surface morphology of IONPs with an average diameter of 50±0.2 nm. Energy-dispersive X-ray spectroscopy (EDS) depicted formation of pure IONPs with 69.84% Fe and 30.16% O2. X-ray diffractometry (XRD) and attenuated total reflectance-fourier transform infrared (ATR-FTIR) validate the crystalline structure, chemical analysis detect the presence of various biomolecular fingerprints in the as synthesized IONPs. UV-visible absorption spectroscopy depicts activity of IONPs under visible light. Thermo-gravimetric analysis (TGA) displayed thermal loss of organic capping around 500°C and confirmed their stabilization. The biosynthesized IONPs revealed promising results in callus induction, shoot regeneration and root induction of chickpea plants. Both chickpea varieties Punjab-Noor 09 and Bittle-98 explants, Embryo axes (EA) and Embryo axes plus adjacent part of cotyledon (EXC) demonstrated dose-dependent response. Among all explants, EXC of Punjab-Noor variety showed the highest callogenesis (96%) and shoot regeneration frequency (88%), while root induction frequency was also increased to 83%. Iron content was quantified in regenerated chickpea varieties through inductively coupled plasma-optical emission spectrometry. The quantity of iron is significantly increased in Punjab-Noor regenerated plants (4.88 mg/g) as compare to control treated plants (2.42 mg/g). We found that IONPs enhance chickpea growth pattern and keep regenerated plantlets infection free by providing an optimum environment for rapid growth and development. Thus, IONPs synthesized through green process can be utilized in tissue culture studies in other important recalcitrant legumes crops.
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