ArticleMolecular and cellular biology1987
Functional domains of SIR4, a gene required for position effect regulation in Saccharomyces cerevisiae.
Article in Molecular and cellular biology, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed.
- Measuring the buffering capacity of gene silencing inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- The Sir4 H-BRCT domain interacts with phospho-proteins to sequester and repress yeast heterochromatin.The EMBO journal · 2019Article
- Genetic basis of octanoic acid resistance in Drosophila sechellia: functional analysis of a fine-mapped region.Molecular ecology · 2017Article
- The Nuts and Bolts of Transcriptionally Silent Chromatin in Saccharomyces cerevisiae.Genetics · 2016Review
- Competition between Heterochromatic Loci Allows the Abundance of the Silencing Protein, Sir4, to Regulate de novo Assembly of Heterochromatin.PLoS genetics · 2015Article
- Regulating repression: roles for the sir4 N-terminus in linker DNA protection and stabilization of epigenetic states.PLoS genetics · 2012Article
- The SCFDia2 ubiquitin E3 ligase ubiquitylates Sir4 and functions in transcriptional silencing.PLoS genetics · 2012Article
- Histone H2B ubiquitylation and H3 lysine 4 methylation prevent ectopic silencing of euchromatic loci important for the cellular response to heat.Molecular biology of the cell · 2011Article
- Clustering heterochromatin: Sir3 promotes telomere clustering independently of silencing in yeast.The Journal of cell biology · 2011Article
- Identification of genes that affect sensitivity to 5-bromodeoxyuridine in the yeast Saccharomyces cerevisiae.Molecular genetics and genomics : MGG · 2010Article
- On gene dosage balance in protein complexes: a comment on Semple JI, Vavouri T, Lehner B. A simple principle concerning the robustness of protein complex activity to changes in gene expression.BMC systems biology · 2009Article
- Subtelomeric elements influence but do not determine silencing levels at Saccharomyces cerevisiae telomeres.Genetics · 2007Article
- Article
- Barrier proteins remodel and modify chromatin to restrict silenced domains.Molecular and cellular biology · 2004Article
- Telomerase-independent proliferation is influenced by cell type in Saccharomyces cerevisiae.Genetics · 2003Article
- Rap1-Sir4 binding independent of other Sir, yKu, or histone interactions initiates the assembly of telomeric heterochromatin in yeast.Genes & development · 2002Article
- Multiple interactions in Sir protein recruitment by Rap1p at silencers and telomeres in yeast.Molecular and cellular biology · 2001Article
- Targeting of the yeast Ty5 retrotransposon to silent chromatin is mediated by interactions between integrase and Sir4p.Molecular and cellular biology · 2001Article
- Sir2p exists in two nucleosome-binding complexes with distinct deacetylase activities.The EMBO journal · 2001Article
- SIR functions are required for the toleration of an unrepaired double-strand break in a dispensable yeast chromosome.Molecular and cellular biology · 2001Article
2 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors.
Funding
Abstract
The product of the Saccharomyces cerevisiae SIR4 gene, in conjunction with at least three other gene products, prevents expression of mating-type genes resident at loci at either end of chromosome III, but not of the same genes resident at the MAT locus in the middle of the chromosome. To address the mechanism of this novel position effect regulation, we have conducted a structural and genetic analysis of the SIR4 gene. We have determined the nucleotide sequence of the gene and found that it encodes a lysine-rich, serine-rich protein of 152 kilodaltons. Expression of the carboxy half of the protein complements a chromosomal nonsense mutation of sir4 but not a complete deletion of the gene. These results suggest that SIR4 protein activity resides in two portions of the molecule, but that these domains need not be covalently linked to execute their biological function. We also found that high-level expression of the carboxy domain of the protein yields dominant derepression of the silent loci. This anti-Sir activity can be reversed by increased expression of the SIR3 gene, whose product is normally also required for maintaining repression of the silent loci. These results are consistent with the hypothesis that SIR3 and SIR4 proteins physically associate to form a multicomponent complex required for repression of the silent mating-type loci.
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